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  • KD/KO Validated

UQCRC2 Polyclonal antibody

UQCRC2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 14742-1-AP

Synonyms

Complex III subunit 2, Core protein II, QCR2, UQCR2, UQCRC2



Tested Applications

Positive WB detected inmouse brain tissue, human colon tissue, mouse colon tissue, mouse heart tissue, mouse placenta tissue, mouse skeletal muscle tissue, SGC-7901 cells, various samples, rat heart tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14742-1-AP targets UQCRC2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UQCRC2 fusion protein Ag6432
Full Name ubiquinol-cytochrome c reductase core protein II
Calculated molecular weight 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC000484
Gene symbol UQCRC2
Gene ID (NCBI) 7385
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UQCRC2(Ubiquinol-cytochrome-c reductase complex core protein 2) is also named as complex III subunit 2,core protein II and belongs to the UQCRC2/QCR2 subfamily. The bc1 complex contains 11 subunits: 3 respiratory subunits (cytochrome b, cytochrome c1 and Rieske/UQCRFS1), 2 core proteins (UQCRC1/QCR1 and UQCRC2/QCR2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and a cleavage product of Rieske/UQCRFS1) and is part of the mitochondrial respiratory chain. This protein distributes in mitochondrion inner membrane, peripheral membrane protein and matrix side.

Protocols

Product Specific Protocols
WB protocol for UQCRC2 antibody 14742-1-APDownload protocol
IHC protocol for UQCRC2 antibody 14742-1-APDownload protocol
IF protocol for UQCRC2 antibody 14742-1-APDownload protocol
IP protocol for UQCRC2 antibody 14742-1-APDownload protocol
FC protocol for UQCRC2 antibody 14742-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,IF

EBioMedicine

Ubiquinol-cytochrome C reductase core protein II promotes tumorigenesis by facilitating p53 degradation.

Authors - Yingyan Han
  • KD Validated
humanWB

Front Cell Dev Biol

Mitochondria in Embryogenesis: An Organellogenesis Perspective.

Authors - Yoan Arribat
humanWB

J Agric Food Chem

Glutathione (GSH) reduces cytotoxicity evoked by patulin (PAT) in HEK293 cells by preventing oxidative damage and mitochondrial apoptotic pathway.

Authors - Xiaorui Wang
mouseWB

Sci Adv

Functional role of respiratory supercomplexes in mice: SCAF1 relevance and segmentation of the Qpool.

Authors - Enrique Calvo
mouseWB

PLoS Genet

AMPK-dependent and -independent coordination of mitochondrial function and muscle fiber type by FNIP1.

Authors - Liwei Xiao
WB

Methods Cell Biol

Analyzing electron transport chain supercomplexes.

Authors - Rebeca Acín-Pérez