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UQCRC2 Polyclonal antibody

UQCRC2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 14742-1-AP

Synonyms

Complex III subunit 2, Core protein II, QCR2, UQCR2, UQCRC2



Tested Applications

Positive WB detected inmouse brain tissue, various samples, human colon tissue, mouse heart tissue, rat heart tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

14742-1-AP targets UQCRC2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, sheep, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UQCRC2 fusion protein Ag6432
Full Name ubiquinol-cytochrome c reductase core protein II
Calculated molecular weight 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC000484
Gene symbol UQCRC2
Gene ID (NCBI) 7385
RRIDAB_2241442
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UQCRC2(Ubiquinol-cytochrome-c reductase complex core protein 2) is also named as complex III subunit 2,core protein II and belongs to the UQCRC2/QCR2 subfamily. The bc1 complex contains 11 subunits: 3 respiratory subunits (cytochrome b, cytochrome c1 and Rieske/UQCRFS1), 2 core proteins (UQCRC1/QCR1 and UQCRC2/QCR2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and a cleavage product of Rieske/UQCRFS1) and is part of the mitochondrial respiratory chain. This protein distributes in mitochondrion inner membrane, peripheral membrane protein and matrix side.

Protocols

Product Specific Protocols
WB protocol for UQCRC2 antibody 14742-1-APDownload protocol
IHC protocol for UQCRC2 antibody 14742-1-APDownload protocol
IF protocol for UQCRC2 antibody 14742-1-APDownload protocol
IP protocol for UQCRC2 antibody 14742-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
sheepWB

Nature

Structure and assembly of the mammalian mitochondrial supercomplex CIII2CIV.

Authors - Irene Vercellino
mouseWB

Nature

Mechanism of super-assembly of respiratory complexes III and IV.

Authors - Sara Cogliati
humanWB

Cell

Mitochondrial Sirtuin Network Reveals Dynamic SIRT3-Dependent Deacetylation in Response to Membrane Depolarization.

Authors - Wen Yang
mouseWB

Cell Metab

Regulation of Mother-to-Offspring Transmission of mtDNA Heteroplasmy.

Authors - Ana Latorre-Pellicer
mouseWB

Nat Cell Biol

AIDA directly connects sympathetic innervation to adaptive thermogenesis by UCP1.

Authors - Meng Shi
mouseWB

Sci Adv

Functional role of respiratory supercomplexes in mice: SCAF1 relevance and segmentation of the Qpool.

Authors - Enrique Calvo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Edward (Verified Customer) (11-15-2022)

Used with 12 ug protein lysate UQCRC2 works well, both with ECL detection and fluorescent secondary antibodies it gives a nice strong band

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:4000
  • Cell Tissue Type: K562 and KCL22