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UQCRC2 Polyclonal antibody
UQCRC2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 14742-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse brain tissue, human colon tissue, mouse colon tissue, mouse heart tissue, mouse placenta tissue, mouse skeletal muscle tissue, SGC-7901 cells, various samples, rat heart tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Positive FC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:12000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 2 publications below |
WB | See 36 publications below |
IHC | See 2 publications below |
IF | See 2 publications below |
Product Information
14742-1-AP targets UQCRC2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, sheep |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | UQCRC2 fusion protein Ag6432 |
Full Name | ubiquinol-cytochrome c reductase core protein II |
Calculated molecular weight | 48 kDa |
Observed molecular weight | 48 kDa |
GenBank accession number | BC000484 |
Gene symbol | UQCRC2 |
Gene ID (NCBI) | 7385 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
UQCRC2(Ubiquinol-cytochrome-c reductase complex core protein 2) is also named as complex III subunit 2,core protein II and belongs to the UQCRC2/QCR2 subfamily. The bc1 complex contains 11 subunits: 3 respiratory subunits (cytochrome b, cytochrome c1 and Rieske/UQCRFS1), 2 core proteins (UQCRC1/QCR1 and UQCRC2/QCR2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and a cleavage product of Rieske/UQCRFS1) and is part of the mitochondrial respiratory chain. This protein distributes in mitochondrion inner membrane, peripheral membrane protein and matrix side.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for UQCRC2 antibody 14742-1-AP | Download protocol |
IHC protocol for UQCRC2 antibody 14742-1-AP | Download protocol |
IF protocol for UQCRC2 antibody 14742-1-AP | Download protocol |
IP protocol for UQCRC2 antibody 14742-1-AP | Download protocol |
FC protocol for UQCRC2 antibody 14742-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
EBioMedicine Ubiquinol-cytochrome C reductase core protein II promotes tumorigenesis by facilitating p53 degradation.
| ||
J Agric Food Chem Glutathione (GSH) reduces cytotoxicity evoked by patulin (PAT) in HEK293 cells by preventing oxidative damage and mitochondrial apoptotic pathway. | ||
Sci Adv Functional role of respiratory supercomplexes in mice: SCAF1 relevance and segmentation of the Qpool. | ||
PLoS Genet AMPK-dependent and -independent coordination of mitochondrial function and muscle fiber type by FNIP1. | ||
Int J Mol Sci Imatinib Regulates miR-483-3p and Mitochondrial Respiratory Complexes in Gastrointestinal Stromal Tumors. |