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UQCRC2 Polyclonal antibody
UQCRC2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 14742-1-AP
Validation Data Gallery
|Positive WB detected in||mouse brain tissue, human colon tissue, mouse colon tissue, mouse heart tissue, mouse placenta tissue, mouse skeletal muscle tissue, SGC-7901 cells, various samples, rat heart tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:2000-1:12000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14742-1-AP targets UQCRC2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, sheep|
|Host / Isotype||Rabbit / IgG|
|Immunogen||UQCRC2 fusion protein Ag6432|
|Full Name||ubiquinol-cytochrome c reductase core protein II|
|Calculated molecular weight||48 kDa|
|Observed molecular weight||48 kDa|
|GenBank accession number||BC000484|
|Gene ID (NCBI)||7385|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
UQCRC2(Ubiquinol-cytochrome-c reductase complex core protein 2) is also named as complex III subunit 2,core protein II and belongs to the UQCRC2/QCR2 subfamily. The bc1 complex contains 11 subunits: 3 respiratory subunits (cytochrome b, cytochrome c1 and Rieske/UQCRFS1), 2 core proteins (UQCRC1/QCR1 and UQCRC2/QCR2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and a cleavage product of Rieske/UQCRFS1) and is part of the mitochondrial respiratory chain. This protein distributes in mitochondrion inner membrane, peripheral membrane protein and matrix side.
|Product Specific Protocols|
|WB protocol for UQCRC2 antibody 14742-1-AP||Download protocol|
|IHC protocol for UQCRC2 antibody 14742-1-AP||Download protocol|
|IF protocol for UQCRC2 antibody 14742-1-AP||Download protocol|
|IP protocol for UQCRC2 antibody 14742-1-AP||Download protocol|
|FC protocol for UQCRC2 antibody 14742-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Structure and assembly of the mammalian mitochondrial supercomplex CIII2CIV.
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Regulation of Mother-to-Offspring Transmission of mtDNA Heteroplasmy.
Nat Cell Biol
AIDA directly connects sympathetic innervation to adaptive thermogenesis by UCP1.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Edward (Verified Customer) (11-15-2022)
Used with 12 ug protein lysate UQCRC2 works well, both with ECL detection and fluorescent secondary antibodies it gives a nice strong band