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UQCRC2 Polyclonal antibody
UQCRC2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 14742-1-AP
Validation Data Gallery
|Positive WB detected in
|mouse brain tissue, various samples, human colon tissue, mouse heart tissue, rat heart tissue
|Positive IP detected in
|Positive IHC detected in
|human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Positive FC detected in
|Western Blot (WB)
|WB : 1:2000-1:12000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:50-1:500
|Flow Cytometry (FC)
|FC : 0.20 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
14742-1-AP targets UQCRC2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat, sheep, zebrafish
|Host / Isotype
|Rabbit / IgG
|UQCRC2 fusion protein Ag6432
|ubiquinol-cytochrome c reductase core protein II
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
UQCRC2(Ubiquinol-cytochrome-c reductase complex core protein 2) is also named as complex III subunit 2,core protein II and belongs to the UQCRC2/QCR2 subfamily. The bc1 complex contains 11 subunits: 3 respiratory subunits (cytochrome b, cytochrome c1 and Rieske/UQCRFS1), 2 core proteins (UQCRC1/QCR1 and UQCRC2/QCR2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and a cleavage product of Rieske/UQCRFS1) and is part of the mitochondrial respiratory chain. This protein distributes in mitochondrion inner membrane, peripheral membrane protein and matrix side.
|Product Specific Protocols
|WB protocol for UQCRC2 antibody 14742-1-AP
|IHC protocol for UQCRC2 antibody 14742-1-AP
|IF protocol for UQCRC2 antibody 14742-1-AP
|IP protocol for UQCRC2 antibody 14742-1-AP
|Click here to view our Standard Protocols
Structure and assembly of the mammalian mitochondrial supercomplex CIII2CIV.
Mechanism of super-assembly of respiratory complexes III and IV.
Mitochondrial Sirtuin Network Reveals Dynamic SIRT3-Dependent Deacetylation in Response to Membrane Depolarization.
Regulation of Mother-to-Offspring Transmission of mtDNA Heteroplasmy.
Nat Cell Biol
AIDA directly connects sympathetic innervation to adaptive thermogenesis by UCP1.
Functional role of respiratory supercomplexes in mice: SCAF1 relevance and segmentation of the Qpool.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Edward (Verified Customer) (11-15-2022)
Used with 12 ug protein lysate UQCRC2 works well, both with ECL detection and fluorescent secondary antibodies it gives a nice strong band