USP33 Recombinant antibody, PBS Only

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  WB analysis using 84958-4-RR (same clone as 84958-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84958-4-RR (USP33 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84958-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84958-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84958-4-RR against Human USP33 were performed. The affinity constant is below 1 pM.

USP33 Uni-rAb^TM Recombinant Antibody for WB, Indirect ELISA

Cat No. 84958-4-PBS

Clone No.242535H2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

242535H2, EC:3.4.19.12, hVDU1, KIAA1097, Ubiquitin carboxyl-terminal hydrolase 33

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Product Information

84958-4-PBS targets USP33 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: USP33 fusion protein Ag14268
• Full Name: ubiquitin specific peptidase 33
• Calculated Molecular Weight: 942 aa, 107 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC016663
• Gene Symbol: USP33
• Gene ID (NCBI): 23032
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8TEY7
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

USP33(Ubiquitin carboxyl-terminal hydrolase 33) is also named as KIAA1097, VDU1 and belongs to the peptidase C19 family. This protein, originally identified as a von Hippel-Lindau tumor suppressor (VHL) protein-interacting deubiquitinating enzyme, binds to the Robo1 receptor, and that USP33 is required for Slit responsiveness in breast cancer cells(PMID:19706539). The localization of USP33 is broadly confined to the secretory pathway, with all variants localizing to endoplasmic reticulum-associated structures(PMID:21801292). It has 3 isoforms produced by alternative splicing.