|Positive WB detected in||A549 cells, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells, LNCaP cells, K-562 cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
66191-1-Ig targets VAPB in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, monkey|
|Host / Isotype||Mouse / IgG1|
|Immunogen||VAPB fusion protein Ag6313|
|Full Name||VAMP (vesicle-associated membrane protein)-associated protein B and C|
|Calculated molecular weight||27 kDa|
|Observed molecular weight||30 kDa|
|GenBank accession number||BC001712|
|Gene ID (NCBI)||9217|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Vesicle-associated membrane protein-associated protein B (VAPB) is an integral membrane protein localized to the endoplasmic reticulum (ER) membrane. VAPB has been implicated in various cellular processes, including ER stress, the unfolded protein response (UPR) and calcium homeostasis regulation. The mutations in the gene of VAPB cause amyotrophic lateral sclerosis 8 (ALS8) and some other related forms of motor neuron disease including late onset spinal muscular atrophy.
Host Vesicle Fusion Protein VAPB Contributes to the Nuclear Egress Stage of Herpes Simplex Virus Type-1 (HSV-1) Replication.
Contact (Thousand Oaks)
Fluorescent tools to analyse peroxisome-ER interactions in mammalian cells.
J Biol Chem
Proteomic mapping by rapamycin-dependent targeting of APEX2 identifies binding partners of VAPB at the inner nuclear membrane.
J Cell Biol
Atlastin 2/3 regulate ER targeting of the ULK1 complex to initiate autophagy.
The ER Contact Proteins VAPA/B Interact with Multiple Autophagy Proteins to Modulate Autophagosome Biogenesis.
CALCOCO1 acts with VAMP-associated proteins to mediate ER-phagy.