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  • KD/KO Validated

VAPB Monoclonal antibody

VAPB Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

4F6A6

Cat no : 66191-1-Ig

Synonyms

ALS8, VAMP associated protein B/C, VAMP B, VAMP B/VAMP C, VAMP C, VAP B, VAP B/VAP C, VAP C, VAPB



Tested Applications

Positive WB detected inA549 cells, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells, LNCaP cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

66191-1-Ig targets VAPB in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, monkey
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen VAPB fusion protein Ag6313
Full Name VAMP (vesicle-associated membrane protein)-associated protein B and C
Calculated molecular weight 27 kDa
Observed molecular weight30 kDa
GenBank accession numberBC001712
Gene symbol VAPB
Gene ID (NCBI) 9217
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Vesicle-associated membrane protein-associated protein B (VAPB) is an integral membrane protein localized to the endoplasmic reticulum (ER) membrane. VAPB has been implicated in various cellular processes, including ER stress, the unfolded protein response (UPR) and calcium homeostasis regulation. The mutations in the gene of VAPB cause amyotrophic lateral sclerosis 8 (ALS8) and some other related forms of motor neuron disease including late onset spinal muscular atrophy.

Protocols

Product Specific Protocols
WB protocol for VAPB antibody 66191-1-IgDownload protocol
IHC protocol for VAPB antibody 66191-1-IgDownload protocol
IF protocol for VAPB antibody 66191-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Cells

Host Vesicle Fusion Protein VAPB Contributes to the Nuclear Egress Stage of Herpes Simplex Virus Type-1 (HSV-1) Replication.

Authors - Natalia Saiz-Ros
human,monkeyWB,IF

Contact (Thousand Oaks)

Fluorescent tools to analyse peroxisome-ER interactions in mammalian cells.

Authors - Alexa Bishop
humanWB

J Biol Chem

Proteomic mapping by rapamycin-dependent targeting of APEX2 identifies binding partners of VAPB at the inner nuclear membrane.

Authors - Christina James
humanWB

J Cell Biol

Atlastin 2/3 regulate ER targeting of the ULK1 complex to initiate autophagy.

Authors - Nan Liu
monkeyWB, CoIP,IF

Curr Biol

The ER Contact Proteins VAPA/B Interact with Multiple Autophagy Proteins to Modulate Autophagosome Biogenesis.

Authors - Yan G Zhao
  • KD Validated
humanWB

EMBO J

CALCOCO1 acts with VAMP-associated proteins to mediate ER-phagy.

Authors - Thaddaeus Mutugi Nthiga