|Positive WB detected in||A549 cells, A431 cells, HeLa cells, HEK-293 cells, mouse spleen tissue, C6 cells|
|Positive IP detected in||mouse spleen tissue|
|Positive IHC detected in||human testis tissue, human heart tissue, human kidney tissue, human lung tissue, human skin cancer tissue, human spleen tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
13472-1-AP targets VASP in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||VASP fusion protein Ag4266|
|Full Name||vasodilator-stimulated phosphoprotein|
|Calculated molecular weight||380 aa, 40 kDa|
|Observed molecular weight||46 kDa, 50 kDa|
|GenBank accession number||BC038224|
|Gene ID (NCBI)||7408|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
VASP belongs to the Ena/VASP family. Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. VASP plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation. Human platelet activation is inhibited by agents such as prostaglandins and NO donors, which elevate cAMP or cGMP levels. VASP is phosphorylated in human platelets in response to both cAMP- and cGMP-elevating agents, and its phosphorylation correlates with platelet inhibition. VASP is located about 92 kb distal to ERCC1 (126380) and about 300 kb proximal to the myotonic dystrophy protein kinase gene. The antibody is specific to VASP.
|Product Specific Protocols|
|WB protocol for VASP antibody 13472-1-AP||Download protocol|
|IHC protocol for VASP antibody 13472-1-AP||Download protocol|
|IF protocol for VASP antibody 13472-1-AP||Download protocol|
|IP protocol for VASP antibody 13472-1-AP||Download protocol|
|FC protocol for VASP antibody 13472-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Cell Death Dis
The novel ZEB1-upregulated protein PRTG induced by Helicobacter pylori infection promotes gastric carcinogenesis through the cGMP/PKG signaling pathway.
Exp Ther Med
Role of vasodilator-stimulated phosphoprotein in RANKL-differentiated murine macrophage RAW264.7 cells: Modulation of NF-κB, c-Fos and NFATc1 transcription factors.
Pathol Res Pract
Silencing lncRNA SNHG6 suppresses proliferation and invasion of breast cancer cells through miR-26a/VASP axis.
The Wnt/β-catenin/VASP positive feedback loop drives cell proliferation and migration in breast cancer.
Nucleotide de novo synthesis increases breast cancer stemness and metastasis via cGMP-PKG-MAPK signaling pathway.
Cortical dynamics during cell motility are regulated by CRL3(KLHL21) E3 ubiquitin ligase.