Validation Data Gallery
|Positive WB detected in
|RAW 264.7 cells, WT and VCP KO U2OS cells, SH-SY5Y cells, HeLa cells
|Positive IHC detected in
|human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|RAW 264.7 cells, HeLa cells, SH-SY5Y cells
|Positive FC detected in
|Western Blot (WB)
|WB : 1:500-1:2000
|IHC : 1:20-1:200
|IF : 1:20-1:200
|Flow Cytometry (FC)
|FC : 0.40 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
60316-1-Ig targets VCP in WB, IHC, IF, FC, ChIP, ELISA applications and shows reactivity with human, mouse samples.
|Host / Isotype
|Mouse / IgG1
|VCP fusion protein Ag1002
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein A purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
VCP(Valosin-containing protein), also known as TER ATPase and 15S Mg2+-ATPase p97 subunit, belongs to the AAA ATPase family. VCP was first identified as a result of attempts to clone a putative peptide hormone called valosin. It was found that the cloned cDNA encoded a ubiquitously expressed 90 kDa cytosolic protein, termed VCP, which showed none of the characteristics of a peptide hormone precursor(PMID:1382975). Defects in VCP are the cause of inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD) and amyotrophic lateral sclerosis type 14 with or without frontotemporal dementia (ALS14). VCP has a calculated molecular weight of 89 kDa and an apparent molecular weight of 90-100 kDa (PMID: 15732117, 1382975).
|Product Specific Protocols
|WB protocol for VCP antibody 60316-1-Ig
|IHC protocol for VCP antibody 60316-1-Ig
|IF protocol for VCP antibody 60316-1-Ig
|FC protocol for VCP antibody 60316-1-Ig
|Click here to view our Standard Protocols
Interactome screening of C9orf72 dipeptide repeats reveals VCP sequestration and functional impairment by polyGA.
Acta Pharmacol Sin
Gossypol, a novel modulator of VCP, induces autophagic degradation of mutant huntingtin by promoting the formation of VCP/p97-LC3-mHTT complex.
Biochim Biophys Acta Gene Regul Mech
GLI1, a novel target of the ER stress regulator p97/VCP, promotes ATF6f-mediated activation of XBP1
Research (Wash D C)
SERBP1 Promotes Stress Granule Clearance by Regulating 26S Proteasome Activity and G3BP1 Ubiquitination and Protects Male Germ Cells from Thermostimuli Damage