VCP Monoclonal antibody

• Featured Product
• KD/KO Validated

VCP Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF, FC, ChIP, ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

2A4B10

Cat no : 60316-1-Ig

Synonyms

15S Mg(2+) ATPase p97 subunit, IBMPFD, p97, TER ATPase, TERA, valosin containing protein, VCP

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Validation Data Gallery

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  WB analysis of HeLa using 60316-1-Ig

  WB result of VCP antibody (60316-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VCP transfected HeLa cells.

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  WB analysis of RAW 264.7 using 60316-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60316-1-Ig (VCP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 60316-1-Ig

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 60316-1-Ig (VCP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 60316-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60316-1-Ig (VCP Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 60316-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60316-1-Ig (VCP Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of RAW 264.7 using 60316-1-Ig

  Immunofluorescent analysis of RAW 264.7 cells using 60316-1-Ig (VCP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

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  IF Staining of SH-SY5Y using 60316-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed SH-SY5Y cells using 60316-1-Ig(VCP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HL-60 using 60316-1-Ig

  1X10^6 HL-60 cells were intracellularly stained with 0.4 ug Anti-Human VCP (60316-1-Ig, Clone:2A4B10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: RAW 264.7 cells, HeLa cells, SH-SY5Y cells
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: RAW 264.7 cells, SH-SY5Y cells
• Positive FC detected in: HL-60 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below
• ChIP: See 1 publications below

Product Information

60316-1-Ig targets VCP in WB, IHC, IF, FC, ChIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: VCP fusion protein Ag1002
• Full Name: valosin-containing protein
• Calculated molecular weight: 89 kDa
• Observed molecular weight: 89 kDa
• GenBank accession number: BC007562
• Gene symbol: VCP
• Gene ID (NCBI): 7415
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VCP(Valosin-containing protein), also known as TER ATPase and 15S Mg2+-ATPase p97 subunit, belongs to the AAA ATPase family. VCP was first identified as a result of attempts to clone a putative peptide hormone called valosin. It was found that the cloned cDNA encoded a ubiquitously expressed 90 kDa cytosolic protein, termed VCP, which showed none of the characteristics of a peptide hormone precursor(PMID:1382975). Defects in VCP are the cause of inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD) and amyotrophic lateral sclerosis type 14 with or without frontotemporal dementia (ALS14). VCP has a calculated molecular weight of 89 kDa and an apparent molecular weight of 90-100 kDa (PMID: 15732117, 1382975).

Publications

Species	Application	Title
human	WB,IF	Brain; Interactome screening of C9orf72 dipeptide repeats reveals VCP sequestration and functional impairment by polyGA.; Authors - Janja Božič ; KD Validated
human	WB	Acta Pharmacol Sin; Gossypol, a novel modulator of VCP, induces autophagic degradation of mutant huntingtin by promoting the formation of VCP/p97-LC3-mHTT complex.; Authors - Xiao-Jing Li
human	WB,ChIP	Biochim Biophys Acta Gene Regul Mech; GLI1, a novel target of the ER stress regulator p97/VCP, promotes ATF6f-mediated activation of XBP1; Authors - Luciana L Almada