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VCP Monoclonal antibody
VCP Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse
Applications
WB, IHC, IF, FC, ChIP, ELISA
Conjugate
Unconjugated
CloneNo.
2A4B10
Cat no : 60316-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | RAW 264.7 cells, HeLa cells, SH-SY5Y cells |
Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | RAW 264.7 cells, SH-SY5Y cells |
Positive FC detected in | HL-60 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 3 publications below |
IF | See 1 publications below |
ChIP | See 1 publications below |
Product Information
60316-1-Ig targets VCP in WB, IHC, IF, FC, ChIP, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | VCP fusion protein Ag1002 |
Full Name | valosin-containing protein |
Calculated molecular weight | 89 kDa |
Observed molecular weight | 89 kDa |
GenBank accession number | BC007562 |
Gene symbol | VCP |
Gene ID (NCBI) | 7415 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
VCP(Valosin-containing protein), also known as TER ATPase and 15S Mg2+-ATPase p97 subunit, belongs to the AAA ATPase family. VCP was first identified as a result of attempts to clone a putative peptide hormone called valosin. It was found that the cloned cDNA encoded a ubiquitously expressed 90 kDa cytosolic protein, termed VCP, which showed none of the characteristics of a peptide hormone precursor(PMID:1382975). Defects in VCP are the cause of inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD) and amyotrophic lateral sclerosis type 14 with or without frontotemporal dementia (ALS14). VCP has a calculated molecular weight of 89 kDa and an apparent molecular weight of 90-100 kDa (PMID: 15732117, 1382975).
Protocols
Product Specific Protocols | |
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WB protocol for VCP antibody 60316-1-Ig | Download protocol |
IHC protocol for VCP antibody 60316-1-Ig | Download protocol |
IF protocol for VCP antibody 60316-1-Ig | Download protocol |
FC protocol for VCP antibody 60316-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Brain Interactome screening of C9orf72 dipeptide repeats reveals VCP sequestration and functional impairment by polyGA.
| ||
Acta Pharmacol Sin Gossypol, a novel modulator of VCP, induces autophagic degradation of mutant huntingtin by promoting the formation of VCP/p97-LC3-mHTT complex. | ||
Biochim Biophys Acta Gene Regul Mech GLI1, a novel target of the ER stress regulator p97/VCP, promotes ATF6f-mediated activation of XBP1 |