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CoraLite®594-conjugated VCP Monoclonal antibody

VCP Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2A4B10

Cat no : CL594-60316

Synonyms

15S Mg(2+) ATPase p97 subunit, IBMPFD, p97, TER ATPase, TERA, valosin containing protein, VCP



Tested Applications

Positive IF detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 60316-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-60316 targets VCP in IF applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen VCP fusion protein Ag1002
Full Name valosin-containing protein
Calculated molecular weight 89 kDa
Observed molecular weight 89 kDa
GenBank accession numberBC007562
Gene symbol VCP
Gene ID (NCBI) 7415
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm/614 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VCP(Valosin-containing protein), also known as TER ATPase and 15S Mg2+-ATPase p97 subunit, belongs to the AAA ATPase family. VCP was first identified as a result of attempts to clone a putative peptide hormone called valosin. It was found that the cloned cDNA encoded a ubiquitously expressed 90 kDa cytosolic protein, termed VCP, which showed none of the characteristics of a peptide hormone precursor(PMID:1382975). Defects in VCP are the cause of inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD) and amyotrophic lateral sclerosis type 14 with or without frontotemporal dementia (ALS14). VCP has a calculated molecular weight of 89 kDa and an apparent molecular weight of 90-100 kDa (PMID: 15732117, 1382975).

Protocols

Product Specific Protocols
IF protocol for CL594 VCP antibody CL594-60316Download protocol
Standard Protocols
Click here to view our Standard Protocols