Vitronectin Polyclonal antibody

Vitronectin Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15833-1-AP

Synonyms

S protein, Serum spreading factor, V75, vitronectin, VN, VNT, VTN



Tested Applications

Positive WB detected inhuman serum tissue, human testis tissue, NIH/3T3 cells
Positive IHC detected inhuman liver tissue, human hepatocirrhosis tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15833-1-AP targets Vitronectin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat, pig, Plasmodium falciparum
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Vitronectin fusion protein Ag8443
Full Name vitronectin
Calculated molecular weight 478 aa, 54 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC005046
Gene symbol VTN
Gene ID (NCBI) 7448
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Vitronectin is a 75-kDa glycoprotein present in blood and in the extracellular matrix (PMID: 10399314). Vitronectin is a multifunctional glycoprotein that mediates cell-to-substrate adhesion, inhibits the cytolytic action of the terminal complement cascade in vitro and binds to several serine protease inhibitors of the serpin family (PMID: 1372588).

Protocols

Product Specific Protocols
WB protocol for Vitronectin antibody 15833-1-APDownload protocol
IHC protocol for Vitronectin antibody 15833-1-APDownload protocol
IF protocol for Vitronectin antibody 15833-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIF,WB

Brain Pathol

Reconstituting neurovascular unit with primary neural stem cells and brain microvascular endothelial cells in three-dimensional matrix.

Authors - Hongjin Wang
humanWB,IF

Sci Rep

Molecular Camouflage of Plasmodium falciparum Merozoites by Binding of Host Vitronectin to P47 Fragment of SERA5.

Authors - Takahiro Tougan
humanWB,IF

J Cell Mol Med

Induction of migration of periodontal ligament cells by selective regulation of integrin subunits.

Authors - Mari Kawamura
humanWB

Proteomics

SILAC-based Quantitative Proteomic Analysis of Secretome between Activated and Reverted Hepatic Stellate Cells.

Authors - Hongyu Zhang
WB

BMC Genomics

The fiber diameter traits of Tibetan cashmere goats are governed by the inherent differences in stress, hypoxic, and metabolic adaptations: an integrative study of proteome and transcriptome.

Authors - Bingru Zhao
Plasmodium falciparumWB,IF

Malar J

The malaria parasite Plasmodium falciparum in red blood cells selectively takes up serum proteins that affect host pathogenicity.

Authors - Takahiro Tougan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Jingwen (Verified Customer) (01-28-2020)

It worked well when I performed the western blot in cancer samples.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Cancer tissue