|Positive WB detected in||human serum tissue, human testis tissue, NIH/3T3 cells|
|Positive IHC detected in||human liver tissue, human hepatocirrhosis tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||NIH/3T3 cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
15833-1-AP targets Vitronectin in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, Plasmodium falciparum, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Vitronectin fusion protein Ag8443|
|Calculated molecular weight||478 aa, 54 kDa|
|Observed molecular weight||75 kDa|
|GenBank accession number||BC005046|
|Gene ID (NCBI)||7448|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Vitronectin is a 75-kDa glycoprotein present in blood and in the extracellular matrix (PMID: 10399314). Vitronectin is a multifunctional glycoprotein that mediates cell-to-substrate adhesion, inhibits the cytolytic action of the terminal complement cascade in vitro and binds to several serine protease inhibitors of the serpin family (PMID: 1372588).
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Jingwen (Verified Customer) (01-28-2020)
It worked well when I performed the western blot in cancer samples.