Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells (Calcein AM, PI Method)

PI:Ex/Em (with DNA)

535/617 nm

Calcein AM:Ex/Em

494/517 nm



Cat no : PF00007

Product Information

Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells (Calcein AM, PI method) provides dual fluorescence staining for the detection of living and dead cells. The two probes in the kit eflect cell viability by measuring intracellular esterase activity and plasma membrane integrity. This kit can be used for fluorescence microscopes, flow cytometers, microplate readers and other fluorescence detection systems. 

This kit can be used for most eukaryotic mammalian samples, but not to fungi and yeast.




 A. Calcein AM (4 mM in anhydrous DMSO)

50 uL

100 uL

 B. Propidium (PI) (1.5 mM in H2O)

250 uL

500 uL

Note: The number of times(T) of this kit is specified according to the usage of 0.5mL working solution for one sample of flow cytometry.


Store at -20°C. Avoid exposure to light. Stable for 24 months after shipment. 


1. Fluorescent dyes all have quenching problems.

2. For your safety and health, please wear lab coats and disposable gloves for operation.



PI: Ex/Em=535/617nm (with DNA)

Cited in Article as

PF00007, Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells (Calcein AM, PI Method), Proteintech, IL, USA



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