Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells (Calcein AM, PI Method)
Cat no : PF00007
Validation Data Gallery
Product Information
Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells (Calcein AM, PI method) provides dual fluorescence staining for the detection of living and dead cells. The two probes in the kit eflect cell viability by measuring intracellular esterase activity and plasma membrane integrity. This kit can be used for fluorescence microscopes, flow cytometers, microplate readers and other fluorescence detection systems.
This kit can be used for most eukaryotic mammalian samples, but not to fungi and yeast.
Components | 150T | 300T |
A. Calcein AM (4 mM in anhydrous DMSO) | 50 μL | 100 μL |
B. Propidium (PI) (1.5 mM in H2O) | 250 μL | 500 μL |
Note: The number of times(T) of this kit is specified according to the usage of 0.5mL working solution for one sample of flow cytometry.
Storage
Store at -20°C. Avoid exposure to light. Stable for 24 months after shipment.
Cautions
1. Fluorescent dyes all have quenching problems.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
Parameters
CalceinAM：Ex/Em=494/517nm
PI: Ex/Em=535/617nm (with DNA)
Cited in Article as
PF00007, Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells (Calcein AM, PI Method), Proteintech, IL, USA
Publications
| Application | Title |
|---|---|
FASEB J HDM induce airway epithelial cell ferroptosis and promote inflammation by activating ferritinophagy in asthma. | |
J Cardiovasc Transl Res CD137 Signal Mediates Cardiac Ischemia-Reperfusion Injury by Regulating the Necrosis of Cardiomyocytes. | |
Drug Des Devel Ther Curcumin- and Cyclopamine-Loaded Liposomes to Enhance Therapeutic Efficacy Against Hepatic Fibrosis. | |
Microb Pathog Luteolin attenuates the pathogenesis of Staphylococcus aureus by interfering with the agr system |