Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells (Calcein AM, PI Method)

PI:Ex/Em (with DNA)

535/617 nm

Calcein AM:Ex/Em

494/517 nm

Application

FC

Cat no : PF00007



Product Information

Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells (Calcein AM, PI method) provides dual fluorescence staining for the detection of living and dead cells. The two probes in the kit eflect cell viability by measuring intracellular esterase activity and plasma membrane integrity. This kit can be used for fluorescence microscopes, flow cytometers, microplate readers and other fluorescence detection systems. 


This kit can be used for most eukaryotic mammalian samples, but not to fungi and yeast.

 Components

150T

300T

 A. Calcein AM (4 mM in anhydrous DMSO)

50 μL

100 μL

 B. Propidium (PI) (1.5 mM in H2O)

250 μL

500 μL

Note: The number of times(T) of this kit is specified according to the usage of 0.5mL working solution for one sample of flow cytometry.

Storage

Store at -20°C. Avoid exposure to light. Stable for 6 months after shipment. 

Cautions

1. Fluorescent dyes all have quenching problems.

2. For your safety and health, please wear lab coats and disposable gloves for operation.

Parameters

CalceinAM:Ex/Em=494/517nm

PI: Ex/Em=535/617nm (with DNA)