Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells (Calcein AM, PI Method)

PI:Ex/Em (with DNA)

535/617 nm

Calcein AM:Ex/Em

494/517 nm

Application

FC

Cat no : PF00007



Product Information

Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells (Calcein AM, PI method) provides dual fluorescence staining for the detection of living and dead cells. The two probes in the kit eflect cell viability by measuring intracellular esterase activity and plasma membrane integrity. This kit can be used for fluorescence microscopes, flow cytometers, microplate readers and other fluorescence detection systems. 


This kit can be used for most eukaryotic mammalian samples, but not to fungi and yeast.

 Components

150T

300T

 A. Calcein AM (4 mM in anhydrous DMSO)

50 μL

100 μL

 B. Propidium (PI) (1.5 mM in H2O)

250 μL

500 μL

Note: The number of times(T) of this kit is specified according to the usage of 0.5mL working solution for one sample of flow cytometry.

Storage

Store at -20°C. Avoid exposure to light. Stable for 24 months after shipment. 

Cautions

1. Fluorescent dyes all have quenching problems.

2. For your safety and health, please wear lab coats and disposable gloves for operation.

Parameters

CalceinAM:Ex/Em=494/517nm

PI: Ex/Em=535/617nm (with DNA)

Cited in Article as

PF00007, Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells (Calcein AM, PI Method), Proteintech, IL, USA

Publications

ApplicationTitle

FASEB J

HDM induce airway epithelial cell ferroptosis and promote inflammation by activating ferritinophagy in asthma.

Authors - Zhaojin Zeng

J Cardiovasc Transl Res

CD137 Signal Mediates Cardiac Ischemia-Reperfusion Injury by Regulating the Necrosis of Cardiomyocytes.

Authors - Yao Xu

Drug Des Devel Ther

Curcumin- and Cyclopamine-Loaded Liposomes to Enhance Therapeutic Efficacy Against Hepatic Fibrosis.

Authors - Ting Zhang

Microb Pathog

Luteolin attenuates the pathogenesis of Staphylococcus aureus by interfering with the agr system

Authors - Qian Yuan