Vinculin Polyclonal antibody

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  WB analysis using 26520-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26520-1-AP (Vinculin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 26520-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 26520-1-AP (Vinculin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 26520-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 26520-1-AP (Vinculin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 26520-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 26520-1-AP (Vinculin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 26520-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 26520-1-AP (Vinculin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skeletal muscle using 26520-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 26520-1-AP (Vinculin Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skeletal muscle using 26520-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 26520-1-AP (Vinculin Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human breast cancer using 26520-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26520-1-AP (Vinculin Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 26520-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26520-1-AP (Vinculin Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human prostate cancer using 26520-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26520-1-AP (Vinculin Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human prostate cancer using 26520-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26520-1-AP (Vinculin Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of A549 using 26520-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Vinculin antibody (26520-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  IF Staining of HepG2 using 26520-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Vinculin antibody (26520-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  FC experiment of HepG2 using 26520-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human Vinculin (26520-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Vinculin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 26520-1-AP

Publications(111)

Reviews (6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

CMD1W, Metavinculin, MV, MVCL, VCL

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, MDCK cells, NIH/3T3 cells, PC-12 cells, mouse heart tissue, rat heart tissue
• Positive IHC detected in: mouse colon tissue, human breast cancer tissue, human prostate cancer tissue, human skeletal muscle tissue, mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, A549 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:60000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 82 publications below
• IF: See 37 publications below

Product Information

26520-1-AP targets Vinculin in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, canine samples.

• Tested Reactivity: human, mouse, rat, canine
• Cited Reactivity: human, mouse, rat, pig, rabbit, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Vinculin fusion protein Ag24942
• Full Name: vinculin
• Calculated Molecular Weight: 1133 aa, 124 kDa
• Observed Molecular Weight: 124 kDa
• GenBank Accession Number: BC039174
• Gene Symbol: Vinculin
• Gene ID (NCBI): 7414
• RRID: AB_2868558
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P18206
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.

Publications

Species	Application	Title
human	WB,IF	Cell Metab; Electron transport chain inhibition increases cellular dependence on purine transport and salvage; Authors - Zheng Wu
human	WB	Blood; Epigenetic Regulation of Non-canonical Menin Targets Modulates Menin Inhibitor Response in Acute Myeloid Leukemia; Authors - Xinyue Zhou
human	WB,IF	ACS Nano; Polyethylenimine-Modified Mesoporous Silica Nanoparticles Induce a Survival Mechanism in Vascular Endothelial Cells via Microvesicle-Mediated Autophagosome Release; Authors - Lu Zhang
human	WB	Mol Cell; Type III CRISPR-mediated flexible RNA excision with engineered guide RNAs; Authors - Yuanfan Sun
mouse	IF	Adv Sci (Weinh); Highly Effective and Noninvasive Near-Infrared Eradication of a Staphylococcus aureus Biofilm on Implants by a Photoresponsive Coating within 20 Min.; Authors - Mu Li
rabbit	WB,IF	Adv Sci (Weinh); Biomimetic Convex Implant for Corneal Regeneration Through 3D Printing; Authors - Yingni Xu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Siavash (Verified Customer) (01-17-2025)

The antibody was used for immunofluorescence staining of 2D cultured cells fixed with 1%PFA and permeabilized with Triton X100. Cells were incubated overnight at +4 degrees celcius. The staining was visible in both epifluorescence microscope and confocal fluorescence microscope. Also, used for immunostaining of formalin-fixed paraffin-embedded (FFPE) skin of mouse. The antigen retrieval was citrate buffer. Primary antibody was incubated overnight at +4 degrees celcius.

• Applications: Immunofluorescence
• Primary Antibody Dilution: Cell: 1:300, Tissue: 1:100
• Cell Tissue Type: Human Primary Epidermal Keratinocytes, Mouse Skin

Vinculin Antibody Immunofluorescence validation (Cell: 1:300, Tissue: 1:100 dilution) in Human Primary Epidermal Keratinocytes, Mouse Skin (Cat no:26520-1-AP)

FH

Sammy (Verified Customer) (12-04-2023)

Very good antibody for detection of vinculin by WB.

• Applications: Western Blot
• Primary Antibody Dilution: 1 in 1000
• Cell Tissue Type: HEKs / HUVEC

Vinculin Antibody Western Blot validation (1 in 1000 dilution) in HEKs / HUVEC (Cat no:26520-1-AP)

FH

Verdiana (Verified Customer) (11-03-2022)

Band at about 140kDa. Great antibody! Bands were visible just after 0.5sec of exposure! Clear bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000 in TBST 5% Milk
• Cell Tissue Type: U2OS

Vinculin Antibody Western Blot validation (1:2000 in TBST 5% Milk dilution) in U2OS (Cat no:26520-1-AP)

FH

Bettina (Verified Customer) (08-03-2022)

Antibody worked well!

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse pancreatic cancer cell line

Vinculin Antibody Western Blot validation (1:1000 dilution) in Mouse pancreatic cancer cell line (Cat no:26520-1-AP)

FH

Silvia (Verified Customer) (02-14-2022)

Good sharp clear band in western blot

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000

FH

Bhuvanasundar (Verified Customer) (10-12-2021)

Immunofluorescence staining with this antibody worked very well.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Epithelial cell