Vinculin Polyclonal antibody

Vinculin Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 26520-1-AP

Synonyms

CMD1W, Metavinculin, MVCL, VCL, Vinculin



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, NIH/3T3 cells
Positive IHC detected inhuman skeletal muscle tissue, human breast cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:300-1:1200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 26520-1-AP is Vinculin Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rabbit
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Vinculin fusion protein Ag24942
Full Name vinculin
Calculated molecular weight 1133 aa, 124 kDa
Observed molecular weight 117 kDa
GenBank accession numberBC039174
Gene symbol VCL
Gene ID (NCBI) 7414
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.

Protocols

Product Specific Protocols
WB protocol for Vinculin antibody 26520-1-APDownload protocol
IHC protocol for Vinculin antibody 26520-1-APDownload protocol
IF protocol for Vinculin antibody 26520-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EBioMedicine

Targeting glutaminase 1 attenuates stemness properties in hepatocellular carcinoma by increasing reactive oxygen species and suppressing Wnt/beta-catenin pathway.

Authors - Binghua Li
humanIF

Mater Today Bio

Vascularized pulp regeneration via injecting simvastatin functionalized GelMA cryogel microspheres loaded with stem cells from human exfoliated deciduous teeth.

Authors - Xiaojing Yuan
humanWB,IF

ACS Nano

Polyethylenimine-Modified Mesoporous Silica Nanoparticles Induce a Survival Mechanism in Vascular Endothelial Cells via Microvesicle-Mediated Autophagosome Release

Authors - Lu Zhang
humanWB,IF

Mol Med Rep

Sustained activation of C3aR in a human podocyte line impairs the morphological maturation of the cells.

Authors - Jing-Min Zheng
humanWB

Bioorg Med Chem

Design, synthesis and biological evaluation of novel 1,3,4,9-tetrahydropyrano[3,4-b]indoles as potential treatment of triple negative breast cancer by suppressing PI3K/AKT/mTOR pathway.

Authors - Jing Qin
humanWB

Prostate

PMID: 30178500

Authors - Zhixiong Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Silvia (Verified Customer) (02-14-2022)

Good sharp clear band in western blot

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:3000
FH

Bhuvanasundar (Verified Customer) (10-12-2021)

Immunofluorescence staining with this antibody worked very well.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Epithelial cell