Vinculin Polyclonal antibody

Vinculin Polyclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat, Canine and More (2)





Cat no : 26520-1-AP


CMD1W, Metavinculin, MVCL, VCL, Vinculin

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, MDCK cells, NIH/3T3 cells, PC-12 cells, mouse heart tissue, rat heart tissue
Positive IHC detected inmouse colon tissue, human breast cancer tissue, human prostate cancer tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:300-1:1200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26520-1-AP targets Vinculin in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat, Canine samples.

Tested Reactivity human, mouse, rat, Canine
Cited Reactivityhuman, mouse, rat, rabbit, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Vinculin fusion protein Ag24942
Full Name vinculin
Calculated Molecular Weight 1133 aa, 124 kDa
Observed Molecular Weight 117 kDa
GenBank Accession NumberBC039174
Gene Symbol VCL
Gene ID (NCBI) 7414
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.


Product Specific Protocols
WB protocol for Vinculin antibody 26520-1-APDownload protocol
IHC protocol for Vinculin antibody 26520-1-APDownload protocol
IF protocol for Vinculin antibody 26520-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Sammy (Verified Customer) (12-04-2023)

Very good antibody for detection of vinculin by WB.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1 in 1000
  • Cell Tissue Type: HEKs / HUVEC
Vinculin Antibody Western Blot validation (1 in 1000 dilution) in HEKs / HUVEC (Cat no:26520-1-AP)

Verdiana (Verified Customer) (11-03-2022)

Band at about 140kDa. Great antibody! Bands were visible just after 0.5sec of exposure! Clear bands.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000 in TBST 5% Milk
  • Cell Tissue Type: U2OS
Vinculin Antibody Western Blot validation (1:2000 in TBST 5% Milk dilution) in U2OS (Cat no:26520-1-AP)

Bettina (Verified Customer) (08-03-2022)

Antibody worked well!

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse pancreatic cancer cell line
Vinculin Antibody Western Blot validation (1:1000 dilution) in Mouse pancreatic cancer cell line (Cat no:26520-1-AP)

Silvia (Verified Customer) (02-14-2022)

Good sharp clear band in western blot

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:3000

Bhuvanasundar (Verified Customer) (10-12-2021)

Immunofluorescence staining with this antibody worked very well.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Epithelial cell