Vinculin Polyclonal antibody
Vinculin Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, Canine and More (2)
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 26520-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293T cells, HeLa cells, HepG2 cells, PC-12 cells, U-87 MG cells, NIH/3T3 cells, MDCK cells, mouse heart tissue, rat heart tissue |
| Positive IHC detected in | human skeletal muscle tissue, human breast cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
| Positive FC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:300-1:1200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 34 publications below |
| IF | See 22 publications below |
Product Information
The immunogen of 26520-1-AP is Vinculin Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat, Canine |
| Cited Reactivity | human, mouse, rat, rabbit, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Vinculin fusion protein Ag24942 |
| Full Name | vinculin |
| Calculated molecular weight | 1133 aa, 124 kDa |
| Observed molecular weight | 117 kDa |
| GenBank accession number | BC039174 |
| Gene symbol | VCL |
| Gene ID (NCBI) | 7414 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Vinculin antibody 26520-1-AP | Download protocol |
| IHC protocol for Vinculin antibody 26520-1-AP | Download protocol |
| IF protocol for Vinculin antibody 26520-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
ACS Nano Polyethylenimine-Modified Mesoporous Silica Nanoparticles Induce a Survival Mechanism in Vascular Endothelial Cells via Microvesicle-Mediated Autophagosome Release | ||
Adv Sci (Weinh) Highly Effective and Noninvasive Near-Infrared Eradication of a Staphylococcus aureus Biofilm on Implants by a Photoresponsive Coating within 20 Min. | ||
Nat Commun Targeting dual-specificity tyrosine phosphorylation-regulated kinase 2 with a highly selective inhibitor for the treatment of prostate cancer. | ||
Small Versatile Protein Coronation Approach with Multiple Depleted Serum for Creating Biocompatible, Precision Nanomedicine. | ||
J Exp Clin Cancer Res RNA-binding protein CCDC137 activates AKT signaling and promotes hepatocellular carcinoma through a novel non-canonical role of DGCR8 in mRNA localization |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Verdiana (Verified Customer) (11-03-2022) | Band at about 140kDa. Great antibody! Bands were visible just after 0.5sec of exposure! Clear bands.
 |
FH Bettina (Verified Customer) (08-03-2022) | Antibody worked well!
 |
FH Silvia (Verified Customer) (02-14-2022) | Good sharp clear band in western blot
|
FH Bhuvanasundar (Verified Customer) (10-12-2021) | Immunofluorescence staining with this antibody worked very well.
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