Vinculin Polyclonal antibody

Vinculin Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, Canine and More (2)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 26520-1-AP

Synonyms

CMD1W, Metavinculin, MVCL, VCL, Vinculin



Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, HepG2 cells, PC-12 cells, U-87 MG cells, NIH/3T3 cells, MDCK cells, mouse heart tissue, rat heart tissue
Positive IHC detected inhuman skeletal muscle tissue, human breast cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:300-1:1200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 26520-1-AP is Vinculin Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat, Canine
Cited Reactivityhuman, mouse, rat, pig, rabbit
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Vinculin fusion protein Ag24942
Full Name vinculin
Calculated molecular weight 1133 aa, 124 kDa
Observed molecular weight 117 kDa
GenBank accession numberBC039174
Gene symbol VCL
Gene ID (NCBI) 7414
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.

Protocols

Product Specific Protocols
WB protocol for Vinculin antibody 26520-1-APDownload protocol
IHC protocol for Vinculin antibody 26520-1-APDownload protocol
IF protocol for Vinculin antibody 26520-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Adv Sci (Weinh)

Highly Effective and Noninvasive Near-Infrared Eradication of a Staphylococcus aureus Biofilm on Implants by a Photoresponsive Coating within 20 Min.

Authors - Mu Li
humanWB

Nat Commun

Targeting dual-specificity tyrosine phosphorylation-regulated kinase 2 with a highly selective inhibitor for the treatment of prostate cancer.

Authors - Kai Yuan
humanIF

Small

Versatile Protein Coronation Approach with Multiple Depleted Serum for Creating Biocompatible, Precision Nanomedicine.

Authors - Chan Wang
humanWB

Clin Transl Med

c-Myb facilitates immune escape of esophageal adenocarcinoma cells through the miR-145-5p/SPOP/PD-L1 axis.

Authors - Lan Zhang
humanWB

Elife

Functional interrogation of HOXA9 regulome in MLLr leukemia via reporter-based CRISPR/Cas9 screen.

Authors - Hao Zhang
humanWB,IF

Elife

Binding blockade between TLN1 and integrin β1 represses triple-negative breast cancer.

Authors - Yixiao Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Verdiana (Verified Customer) (11-03-2022)

Band at about 140kDa. Great antibody! Bands were visible just after 0.5sec of exposure! Clear bands.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000 in TBST 5% Milk
  • Cell Tissue Type: U2OS
Vinculin Antibody Western Blot validation (1:2000 in TBST 5% Milk dilution) in U2OS (Cat no:26520-1-AP)
FH

Bettina (Verified Customer) (08-03-2022)

Antibody worked well!

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse pancreatic cancer cell line
Vinculin Antibody Western Blot validation (1:1000 dilution) in Mouse pancreatic cancer cell line (Cat no:26520-1-AP)
FH

Silvia (Verified Customer) (02-14-2022)

Good sharp clear band in western blot

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:3000
FH

Bhuvanasundar (Verified Customer) (10-12-2021)

Immunofluorescence staining with this antibody worked very well.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Epithelial cell