Vinculin Polyclonal antibody

Vinculin Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 26520-1-AP

Synonyms

CMD1W, Metavinculin, MVCL, VCL, Vinculin



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, NIH/3T3 cells
Positive IHC detected inhuman skeletal muscle tissue, human breast cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:300-1:1200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 26520-1-AP is Vinculin Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, rabbit
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Vinculin fusion protein Ag24942
Full Name vinculin
Calculated molecular weight 1133 aa, 124 kDa
Observed molecular weight 117 kDa
GenBank accession numberBC039174
Gene symbol VCL
Gene ID (NCBI) 7414
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.

Protocols

Product Specific Protocols
WB protocol for Vinculin antibody 26520-1-APDownload protocol
IHC protocol for Vinculin antibody 26520-1-APDownload protocol
IF protocol for Vinculin antibody 26520-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EBioMedicine

Targeting glutaminase 1 attenuates stemness properties in hepatocellular carcinoma by increasing reactive oxygen species and suppressing Wnt/beta-catenin pathway.

Authors - Binghua Li
humanWB,IF

Mol Med Rep

Sustained activation of C3aR in a human podocyte line impairs the morphological maturation of the cells.

Authors - Jing-Min Zheng
humanWB

Prostate

PMID: 30178500

Authors - Zhixiong Chen
mouseWB

Front Nutr

The Essential Role of Selenoproteins in the Resolution of Citrobacter rodentium-Induced Intestinal Inflammation.

Authors - Shaneice K Nettleford
humanIF

Cell Commun Signal

FAK displacement from focal adhesions: a promising strategy to target processes implicated in cancer progression and metastasis.

Authors - Ioanna Antoniades
humanIF

Front Oncol

MLK3 Is Associated With Poor Prognosis in Patients With Glioblastomas and Actin Cytoskeleton Remodeling in Glioblastoma Cells.

Authors - Yan Zhu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Bhuvanasundar (Verified Customer) (10-12-2021)

Immunofluorescence staining with this antibody worked very well.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Epithelial cell