Validation Data Gallery
|Positive WB detected in||HEK-293T cells, HeLa cells, HepG2 cells, PC-12 cells, U-87 MG cells, NIH/3T3 cells, MDCK cells, mouse heart tissue, rat heart tissue|
|Positive IHC detected in||human skeletal muscle tissue, human breast cancer tissue, human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:300-1:1200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 26520-1-AP is Vinculin Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat, Canine|
|Cited Reactivity||human, mouse, rat, pig, rabbit|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Vinculin fusion protein Ag24942|
|Calculated molecular weight||1133 aa, 124 kDa|
|Observed molecular weight||117 kDa|
|GenBank accession number||BC039174|
|Gene ID (NCBI)||7414|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Verdiana (Verified Customer) (11-03-2022)
Band at about 140kDa. Great antibody! Bands were visible just after 0.5sec of exposure! Clear bands.
Bettina (Verified Customer) (08-03-2022)
Antibody worked well!
Silvia (Verified Customer) (02-14-2022)
Good sharp clear band in western blot
Bhuvanasundar (Verified Customer) (10-12-2021)
Immunofluorescence staining with this antibody worked very well.