CoraLite® Plus 488-conjugated Vinculin Polyclonal antibody

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  IF Staining of HepG2 using CL488-26520

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 Vinculin antibody (CL488-26520) at dilution of 1:200, CL594-phalloidin (red).

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  IF Staining of A549 using CL488-26520

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 Vinculin antibody (CL488-26520) at dilution of 1:200, CL594-Phalloidin (red).

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  FC experiment of HepG2 using CL488-26520

  1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Vinculin (CL488-26520) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Vinculin Polyclonal Antibody for IF/ICC, FC (Intra)

Cat No. CL488-26520

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Synonyms

VCL, MVCL, MV, Metavinculin, CMD1W

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells, A549 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-26520 targets Vinculin in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag24942

  Product name: Recombinant human Vinculin protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 777-1066 aa of BC039174

  Sequence: PKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

• Full Name: vinculin
• Calculated Molecular Weight: 1133 aa, 124 kDa
• Observed Molecular Weight: 117 kDa
• GenBank Accession Number: BC039174
• Gene Symbol: Vinculin
• Gene ID (NCBI): 7414
• RRID: AB_2934418
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P18206
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.