WAPL Recombinant monoclonal antibody, PBS Only

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  WB analysis using 84877-5-RR (same clone as 84877-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84877-5-RR (WAPL antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84877-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 84877-5-RR (same clone as 84877-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84877-5-RR (WAPL antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84877-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 84877-5-RR (same clone as 84877-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84877-5-RR (WAPL antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84877-5-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84877-5-RR (same clone as 84877-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using WAPL antibody (84877-5-RR, Clone: 242443C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84877-5-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 84877-5-RR (same clone as 84877-5-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug WAPL Recombinant antibody (84877-5-RR, Clone:242443C1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 84877-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84877-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84877-5-RR against Human WAPL were performed. The affinity constant is 0.23 nM.

WAPL Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 84877-5-PBS

Clone No.242443C1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

FOE, WAPL/FOE, 242443C1, KIAA0261, WAPAL

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Product Information

84877-5-PBS targets WAPL in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag9585

  Product name: Recombinant human WAPL; WAPAL protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-402 aa of BC017393

  Sequence: MDLDRASLDLMIRLLELEQDASSAKLLNEKDMNKIKEKIRRLCETVHNKHLDLENITTGHLAMETLLSLTSKRAGDWFKEELRLLGGLDHIVDKVKECVDHLSRDEDEEKLVASLWGAERCLRVLESVTVHNPENQSYLIAYKDSQLIVSSAKALQHCEELIQQYNRAEDSICLADSKPLPHQNVTNHVGKAVEDCMRAIIGVLLNLTNDNEWGSTKTGEQDGLIGTALNCVLQVPKYLPQEQRFDIRVLLFLERERAAQLAESKTDELIKDAPTTQHDKSGEWQETSGEIQWVSTEKTDGTEEKHKKEEEDEELDLNKALQHAGKHMEDCIVASYTALLLGCLCQESPINVTTVREYLPEGDFSIMTEMLKKFLSFMNLTCAVGTTGQKSISRVIEYLEHC

• Full Name: wings apart-like homolog (Drosophila)
• Calculated Molecular Weight: 142 kDa
• Observed Molecular Weight: 160-180 kDa
• GenBank Accession Number: BC017393
• Gene Symbol: WAPL
• Gene ID (NCBI): 23063
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q7Z5K2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The wings apart-like (WAPL, or WAPAL) protein was originally identified as a gene product that potentially regulates heterochromatin organization in Drosophila melanogaster. Human WAPL is a cohesin-binding protein that promotes sister-chromatid resolution in mitotic prophase. Overexpression of WAPL disturbs mitosis and cytokinesis, and contributes to tumor progression by induction of chromosomal instability. WAPAL has a predicted molecular weight of 140 kDa, however, as a result of its extensive phosphorylation it separates on a SDS gel as a band of approximately 160-180 kDa.