WASF1 Polyclonal antibody

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  WB analysis using 32584-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32584-1-AP (WASF1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 32584-1-AP

  IP result of anti-WASF1 (IP:32584-1-AP, 4ug; Detection:32584-1-AP 1:3000) with HEK-293 cells lysate 1800 ug.

WASF1 Polyclonal Antibody for WB, IP, ELISA

Cat No. 32584-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

Actin-binding protein WASF1, KIAA0269, Protein WAVE-1, SCAR1, Verprolin homology domain-containing protein 1

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IP detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32584-1-AP targets WASF1 in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WASF1 fusion protein Ag38505
• Full Name: WAS protein family, member 1
• Calculated Molecular Weight: 62kDa，559aa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: NM_003931.2
• Gene Symbol: WASF1
• Gene ID (NCBI): 8936
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q92558
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WASF1 (Wiskott-Aldrich Syndrome Protein Family Member 1) is a gene encoding an actin-binding protein that belongs to the Wiskott-Aldrich syndrome protein family. It is primarily involved in regulating the reorganization of the actin cytoskeleton in cells, and is essential for processes such as cell morphology, motility, and signaling. WASF1 promotes actin polymerization by interacting with the Arp2/3 complex, thereby affecting cell deformation and migration. WASF1 is expressed in a variety of cell types, including tissues such as brain and testis. Its aberrant expression is associated with a variety of diseases, such as tumor invasion and metastasis.