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WFS1 Polyclonal antibody

WFS1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, coIP, ELISA

Conjugate

Unconjugated

Cat no : 11558-1-AP

Synonyms

WFRS, WFS, WFS1, Wolfram syndrome 1 (wolframin), WOLFRAMIN



Tested Applications

Positive WB detected inSH-SY5Y cells, HEK-293 cells
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

11558-1-AP targets WFS1 in WB, IHC, IF, coIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WFS1 fusion protein Ag2114
Full Name Wolfram syndrome 1 (wolframin)
Calculated molecular weight 890 aa, 100 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC030130
Gene symbol WFS1
Gene ID (NCBI) 7466
RRIDAB_2216046
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Wolfram syndrome protein (WFS1), also called wolframin, is a transmembrane protein, which is located primarily in the endoplasmic reticulum and its expression is induced in response to ER stress, partially through transcriptional activation. ER localization suggests that WFS1 protein has physiological functions in membrane trafficking, secretion, processing and/or regulation of ER calcium homeostasis. It is ubiquitously expressed with highest levels in brain, pancreas, heart, and insulinoma beta-cell lines. Mutations of the WFS1 gene are responsible for two hereditary diseases, autosomal recessive Wolfram syndrome and autosomal dominant low frequency sensorineural hearing loss.

Protocols

Product Specific Protocols
WB protocol for WFS1 antibody 11558-1-APDownload protocol
IHC protocol for WFS1 antibody 11558-1-APDownload protocol
IF protocol for WFS1 antibody 11558-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Science

Monosomes actively translate synaptic mRNAs in neuronal processes.

Authors - Anne Biever
mouseIF

Cell

A Hippocampus-Accumbens Tripartite Neuronal Motif Guides Appetitive Memory in Space.

Authors - Stéphanie Trouche
mouseIF

Nature

The hippocampal CA2 region is essential for social memory.

Authors - Frederick L Hitti
mouseIF

Science

Island cells control temporal association memory.

Authors - Takashi Kitamura
mouseIF

Cell

A Map-like Micro-Organization of Grid Cells in the Medial Entorhinal Cortex.

Authors - Yi Gu
mouseIF

Cell

Distinct Neural Circuits for the Formation and Retrieval of Episodic Memories.

Authors - Dheeraj S Roy

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Lana (Verified Customer) (05-29-2020)

SDS-PAGE: 15 ug/ul RIPA lysate of whole brain tissue or mitochondrial fraction. 4-12% Bis-tris gradient gel.Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4CBlocking: SEA Block Blocking Buffer 1hPrimary Ab: O/N incubation at 4C, 1:1000Secondary Ab: IRDye 800CW Goat anti-Rabbit, 1:15000Lines of WB: 1 – protein ladder, 2 – whole brain tissue lysate, 3 – mitochondrial fraction lysate.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Whole brain tissue (human), mitochondrial fraction from brain tissue (human).
WFS1 Antibody Western Blot validation (1:1000 dilution) in Whole brain tissue (human), mitochondrial fraction from brain tissue (human). (Cat no:11558-1-AP)