Validation Data Gallery
|Positive WB detected in||SH-SY5Y cells, HEK-293 cells|
|Positive IHC detected in||rat brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse brain tissue|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:200-1:800|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11558-1-AP targets WFS1 in WB, IHC, IF, coIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||WFS1 fusion protein Ag2114|
|Full Name||Wolfram syndrome 1 (wolframin)|
|Calculated molecular weight||890 aa, 100 kDa|
|Observed molecular weight||100 kDa|
|GenBank accession number||BC030130|
|Gene ID (NCBI)||7466|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Wolfram syndrome protein (WFS1), also called wolframin, is a transmembrane protein, which is located primarily in the endoplasmic reticulum and its expression is induced in response to ER stress, partially through transcriptional activation. ER localization suggests that WFS1 protein has physiological functions in membrane trafficking, secretion, processing and/or regulation of ER calcium homeostasis. It is ubiquitously expressed with highest levels in brain, pancreas, heart, and insulinoma beta-cell lines. Mutations of the WFS1 gene are responsible for two hereditary diseases, autosomal recessive Wolfram syndrome and autosomal dominant low frequency sensorineural hearing loss.
Proximodistal Organization of the CA2 Hippocampal Area.
Alpha-synuclein suppresses mitochondrial protease ClpP to trigger mitochondrial oxidative damage and neurotoxicity.
Dorsal CA1 interneurons contribute to acute stress-induced spatial memory deficits.
RGS14 Restricts Plasticity in Hippocampal CA2 by Limiting Postsynaptic Calcium Signaling.
Serotonin receptor 2c-expressing cells in the ventral CA1 control attention via innervation of the Edinger-Westphal nucleus.
Acta Neuropathol Commun
The transcriptional coactivator and histone acetyltransferase CBP regulates neural precursor cell development and migration.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Lana (Verified Customer) (05-29-2020)
SDS-PAGE: 15 ug/ul RIPA lysate of whole brain tissue or mitochondrial fraction. 4-12% Bis-tris gradient gel.Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4CBlocking: SEA Block Blocking Buffer 1hPrimary Ab: O/N incubation at 4C, 1:1000Secondary Ab: IRDye 800CW Goat anti-Rabbit, 1:15000Lines of WB: 1 – protein ladder, 2 – whole brain tissue lysate, 3 – mitochondrial fraction lysate.