WIPI1 Polyclonal antibody, PBS Only

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  WB analysis of SW 1990 using 25204-1-AP (same clone as 25204-1-PBS)

  SW 1990 cells were subjected to SDS PAGE followed by western blot with 25204-1-AP (WIPI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25204-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 25204-1-AP (same clone as 25204-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25204-1-AP (WIPI1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25204-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 25204-1-AP (same clone as 25204-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25204-1-AP (WIPI1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25204-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SW 1990 using 25204-1-AP (same clone as 25204-1-PBS)

  SW 1990 cells were subjected to SDS PAGE followed by western blot with 25204-1-AP (WIPI1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25204-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 25204-1-AP (same clone as 25204-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 25204-1-AP (WIPI1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25204-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 25204-1-AP (same clone as 25204-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 25204-1-AP (WIPI1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25204-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 25204-1-AP (same clone as 25204-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25204-1-AP (WIPI1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25204-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 25204-1-AP (same clone as 25204-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25204-1-AP (WIPI1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25204-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

WIPI1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 25204-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

WIPI-1, WIPI 49 kDa, WIPI 1, WD repeat domain phosphoinositide-interacting protein 1, Atg18 protein homolog

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Product Information

25204-1-PBS targets WIPI1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WIPI1 fusion protein Ag18461
• Full Name: WD repeat domain, phosphoinositide interacting 1
• Calculated Molecular Weight: 446 aa, 49 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC039867
• Gene Symbol: WIPI1
• Gene ID (NCBI): 55062
• RRID: AB_2879958
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5MNZ9
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

WIPI1, or WD repeat domain, phosphoinositide-interacting protein 1, is a member of the WD40 repeat family of proteins, which are key components of many essential biological functions. WIPI1 is involved in the regulation of autophagy, a cellular process critical for maintaining cellular integrity and recycling intracellular proteins, lipids, and organelles. WIPI1 is characterized by its 7-bladed β-propeller structure and contains a conserved motif for interaction with phospholipids, specifically binding to phosphoinositides. This interaction is crucial for its function in autophagy, where it acts as an effector of phosphatidylinositol 3-phosphate (PtdIns3P). WIPI1 and WIPI2 are among the first proteins to be recruited during autophagy, with WIPI2 interacting with components of the ULK1/2 complex and PtdIns3P, tethering the phagophore to the endoplasmic reticulum (ER). WIPI1 supports the function of WIPI2 in recruiting the ATG16L complex, which is essential for LC3 lipidation and autophagosome formation.