XBP1S-specific Polyclonal antibody

• 

  WB analysis using 24868-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24868-1-AP (XBP1S-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24868-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24868-1-AP (XBP1S-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24868-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24868-1-AP (XBP1S-specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 24868-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 24868-1-AP (XBP1S-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 24868-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24868-1-AP (XBP1S antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 24868-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24868-1-AP (XBP1S antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 24868-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24868-1-AP (XBP1S antibody at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 24868-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 24868-1-AP (XBP1S antibody at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 24868-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 24868-1-AP (XBP1S antibody at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 24868-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24868-1-AP (XBP1S-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 24868-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24868-1-AP (XBP1S antibody at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 24868-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24868-1-AP (XBP1S antibody at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 24868-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24868-1-AP (XBP1S antibody at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HepG2 using 24868-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human XBP1S-specific (24868-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 90% MeOH and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

XBP1S-specific Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Cat No. 24868-1-AP

Publications(96)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, FC (Intra), ELISA

Synonyms

XBP1, Tax-responsive element-binding protein 5, TREB-5, X box binding protein 1, X-box-binding protein 1

---

Tested Applications

• Positive WB detected in: HEK-293T cells, MCF-7 cells, mouse liver tissue, rat liver tissue
• Positive IHC detected in: human breast cancer tissue, human colon cancer tissue, human liver cancer tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 90 publications below
• IHC: See 9 publications below
• IF: See 11 publications below

Product Information

24868-1-AP targets XBP1S-specific in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: XBP1S-specific fusion protein Ag21703
• Full Name: X-box binding protein 1
• Calculated Molecular Weight: 261 aa, 29 kDa
• Observed Molecular Weight: 45-65 kDa
• GenBank Accession Number: BC000938
• Gene Symbol: XBP1
• Gene ID (NCBI): 7494
• RRID: AB_2879766
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P17861
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

XBP1, also named as XBP2, belongs to the bZIP family. The X-box-binding protein-1 (XBP1) is a transcriptional regulator of the ER stress response that lies downstream of inositol-requiring enzyme 1 (IRE1α) activation (PMID: 14559994). XBP1 localizes in the nucleus. As a transcription factor, it is essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response. XBP1 has an association with major affective disorder. The unspliced XBP1U isoform is composed of 261 amino acid residues, and the spliced XBP1S isoform is composed of 376 amino acid residues. The calculated molecular weight of protein generated from the unspliced XBP1 isoform XBP1U mRNA is 33 kDa, and protein generated from spliced XBP1 isoform XBP1S mRNA is 40 kDa, and shows at 45-65 kDa range. This antibody specially recognizes the XBP1S isoform.

Publications

Species	Application	Title
mouse	WB,IF	Nat Commun; A nanoemulsion targeting adipose hypertrophy and hyperplasia shows anti-obesity efficiency in female mice; Authors - Yichao Lu
mouse	IF	Acta Pharm Sin B; The substitution of SERCA2 redox cysteine 674 promotes pulmonary vascular remodeling by activating IRE1α/XBP1s pathway.; Authors - Weimin Yu
mouse	WB	Nat Commun; Activation of GCN2/ATF4 signals in amygdalar PKC-δ neurons promotes WAT browning under leucine deprivation.; Authors - Feixiang Yuan
human	WB	Cancer Lett; LIMK1 m6A-RNA methylation recognized by YTHDC2 induces 5-FU chemoresistance in colorectal cancer via endoplasmic reticulum stress and stress granule formation; Authors - Lu Chen
	WB	Diabetes; Lipid Droplets Protect Human β-Cells From Lipotoxicity-Induced Stress and Cell Identity Changes.; Authors - Xin Tong
human	WB	Oxid Med Cell Longev; Drp1-Mediated Mitochondrial Metabolic Dysfunction Inhibits the Tumor Growth of Pituitary Adenomas.; Authors - Kexia Fan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Kevin (Verified Customer) (08-19-2019)

Good antibody. Clear and sharp bands on liver tissue from mouse.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse liver tissue