XBP1S-specific Polyclonal antibody

• Featured Product
• KD/KO Validated

XBP1S-specific Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

48

Reviews (1)

Cat no : 24868-1-AP

Synonyms

TREB5, X box binding protein 1, XBP 1, XBP1, XBP1S, XBP1S-specific, XBP2

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Validation Data Gallery

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  WB analysis using 24868-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24868-1-AP (XBP1S-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24868-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24868-1-AP (XBP1S-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24868-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24868-1-AP (XBP1S-specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 24868-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 24868-1-AP (XBP1S-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 24868-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24868-1-AP (XBP1S antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 24868-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 24868-1-AP (XBP1S antibody at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 24868-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 24868-1-AP (XBP1S antibody at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 24868-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24868-1-AP (XBP1S antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 24868-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24868-1-AP (XBP1S antibody at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 24868-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24868-1-AP (XBP1S antibody at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 24868-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24868-1-AP (XBP1S antibody at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 24868-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24868-1-AP (XBP1S antibody at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 24868-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24868-1-AP (XBP1S-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293T cells, MCF-7 cells, mouse liver tissue, rat liver tissue
• Positive IHC detected in: human breast cancer tissue, human colon cancer tissue, human liver cancer tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 44 publications below
• IHC: See 6 publications below
• IF: See 4 publications below

Product Information

24868-1-AP targets XBP1S-specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: XBP1S-specific fusion protein Ag21703
• Full Name: X-box binding protein 1
• Calculated molecular weight: 261 aa, 29 kDa
• Observed molecular weight: 55 kDa
• GenBank accession number: BC000938
• Gene symbol: XBP1
• Gene ID (NCBI): 7494
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

XBP1, also named as XBP2, belongs to the bZIP family. The X-box-binding protein-1 (XBP1) is a transcriptional regulator of the ER stress response that lies downstream of inositol-requiring enzyme 1 (IRE1α) activation (PMID: 14559994). XBP1 localizes in the nucleus. As a transcription factor, it is essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response. XBP1 has an association with major affective disorder. The unspliced XBP1U isoform is composed of 261 amino acid residues, and the spliced XBP1S isoform is composed of 376 amino acid residues. The calculated molecular weight of protein generated from the unspliced XBP1 isoform XBP1U mRNA is 33 kDa, and protein generated from spliced XBP1 isoform XBP1S mRNA is 54 kDa. This antibody specially recognizes the XBP1S isoform.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Activation of GCN2/ATF4 signals in amygdalar PKC-δ neurons promotes WAT browning under leucine deprivation.; Authors - Feixiang Yuan
	WB	Diabetes; Lipid Droplets Protect Human β-Cells From Lipotoxicity-Induced Stress and Cell Identity Changes.; Authors - Xin Tong
mouse	WB	J Cell Physiol; Endoplasmic reticulum stress promotes sepsis-induced muscle atrophy via activation of STAT3 and Smad3; Authors - Yingfang Zheng
human	WB	Cell Mol Life Sci; Neuronal microRNAs safeguard ER Ca2+ homeostasis and attenuate the unfolded protein response upon stress.; Authors - Maria Paschou
human	WB,IHC	Front Cell Dev Biol; Obesity impacts placental function through activation of p-IRE1a-XBP1s signaling; Authors - Wei-Bin Shen
mouse	WB	Transl Stroke Res; Melatonin Offers Dual-Phase Protection to Brain Vessel Endothelial Cells in Prolonged Cerebral Ischemia-Recanalization Through Ameliorating ER Stress and Resolving Refractory Stress Granule; Authors - Danli Lu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kevin (Verified Customer) (08-19-2019)

Good antibody. Clear and sharp bands on liver tissue from mouse.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse liver tissue