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XBP1S-specific Polyclonal antibody

XBP1S-specific Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24868-1-AP

Synonyms

TREB5, X box binding protein 1, XBP 1, XBP1, XBP1S, XBP1S-specific, XBP2



Tested Applications

Positive WB detected inMDA-MB-453s cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue, human liver cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 24868-1-AP is XBP1S-specific Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen XBP1S-specific fusion protein Ag21703
Full Name X-box binding protein 1
Calculated molecular weight 261 aa, 29 kDa
Observed molecular weight 55 kDa, 30 kDa
GenBank accession numberBC000938
Gene symbol XBP1
Gene ID (NCBI) 7494
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

X-box-binding protein 1 (XBP1), also named as TREB5, is a 261 amino acid protein, which contains one bZIP domain and belongs to the bZIP family. XBP1 localizes in the nucleus and as a transcription factor is essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response. XBP1 has an association with major affective disorder. The molecular weight of protein generated from the spliced XBP1 mRNA is 54 kDa and protein generated from unspliced Xbp1 mRNA is 33 kDa. This antibody 24868-1-AP specially recognizes isoform XBP-1S.

Protocols

Product Specific Protocols
WB protocol for XBP1S-specific antibody 24868-1-APDownload protocol
IHC protocol for XBP1S-specific antibody 24868-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Mol Med Rep

Overexpression of lncRNA Dancr inhibits apoptosis and enhances autophagy to protect cardiomyocytes from endoplasmic reticulum stress injury via sponging microRNA-6324.

Authors - Jiong Li
humanWB

Cancer Biol Ther

The novel autophagy inhibitor elaiophylin exerts antitumor activity against multiple myeloma with mutant TP53 in part through endoplasmic reticulum stress-induced apoptosis.

Authors - Wang Gaoxiang
ratWB

Toxicol Lett

Methamphetamine mediates apoptosis of vascular smooth muscle cells via the chop-related endoplasmic reticulum stress pathway.

Authors - Xiaohui Tan
ratWB,IHC

J Ethnopharmacol

Compound Tongluo Decoction inhibits endoplasmic reticulum stress-induced ferroptosis and promoted angiogenesis by activating the Sonic Hedgehog pathway in cerebral infarction.

Authors - Zhen Hui
humanWB

Biomed Pharmacother

Asiatic acid ameliorates acute hepatic injury by reducing endoplasmic reticulum stress and triggering hepatocyte autophagy.

Authors - Xiaohong Pang
mouse,humanWB

Front Pharmacol

Salvianolic Acid B Protects Against Fatty Acid-Induced Renal Tubular Injury via Inhibition of Endoplasmic Reticulum Stress.

Authors - Xiaoyi Mai

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Kevin (Verified Customer) (08-19-2019)

Good antibody. Clear and sharp bands on liver tissue from mouse.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse liver tissue