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  • KD/KO Validated

YTHDF1 Polyclonal antibody

YTHDF1 Polyclonal Antibody for IF, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (3)





Cat no : 17479-1-AP


C20orf21, DACA 1, YTH domain family protein 1, YTH domain family, member 1, YTHDF1

Tested Applications

Positive IP detected inmouse brain tissue
Positive IHC detected inhuman kidney tissue, human hypothalamus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

17479-1-AP targets YTHDF1 in WB, RIP, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, monkey, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen YTHDF1 fusion protein Ag11509
Full Name YTH domain family, member 1
Calculated molecular weight 559 aa, 61 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC050284
Gene symbol YTHDF1
Gene ID (NCBI) 54915
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

YTHDF1, also named as YTH domain-containing family protein 1 or C20orf21, is a 559 amino acid protein, which localizes in the cytoplasm. YTHDF1 specifically recognizes and binds N6-methyladenosine (m6A)-containing mRNAs, and promotes mRNA translation efficiency. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. YTHDF1 acts as a regulator of mRNA translation efficiency: promotes ribosome loading to m6A-containing mRNAs and interacts with translation initiation factors eIF3 (EIF3A or EIF3B) to facilitate translation initiation. YTHDF1 exists two isoform and calculated molecular weight of isoforms are 61 kDa and 21 kDa.


Product Specific Protocols
WB protocol for YTHDF1 antibody 17479-1-APDownload protocol
IHC protocol for YTHDF1 antibody 17479-1-APDownload protocol
IF protocol for YTHDF1 antibody 17479-1-APDownload protocol
IP protocol for YTHDF1 antibody 17479-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Anti-tumour immunity controlled through mRNA m6A methylation and YTHDF1 in dendritic cells.

Authors - Dali Han
  • KO Validated

Nucleic Acids Res

The m6A reader YTHDF1 regulates axon guidance through translational control of Robo3.1 expression.

Authors - Mengru Zhuang
  • KO Validated

Cell Death Dis

m6A methylation controls pluripotency of porcine induced pluripotent stem cells by targeting SOCS3/JAK2/STAT3 pathway in a YTHDF1/YTHDF2-orchestrated manner.

Authors - Ruifan Wu


Dynamic m6A mRNA methylation reveals the role of METTL3-m6A-CDCP1 signaling axis in chemical carcinogenesis.

Authors - Fan Yang
  • KD Validated

Int J Obes (Lond)

mRNA m6A plays opposite role in regulating UCP2 and PNPLA2 protein expression in adipocytes.

Authors - Xinxia Wang

Genes Dev

PMID: 30463905

Authors - Rosa M Rubio


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Sarah (Verified Customer) (01-05-2021)

Works great in WB at 1/1000 (5% Milk TBS Tween). Tested on HeLa and 293T lysates;

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HeLa and HEL293T

Hannah (Verified Customer) (08-14-2020)

Band observed by WB of correct size that is reduced with siRNA knockdown but required long exposure or Pico supersignal ECL for a good signal from fibroblast lysates

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1/1000 for WB; 1/100 for IF
  • Cell Tissue Type: human fibroblasts

Biao (Verified Customer) (03-11-2020)

This antibody is very specific and good quality.

  • Applications: Western Blot,
  • Cell Tissue Type: hct-116

Zizhao (Verified Customer) (12-03-2018)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HaCaT/human keratinocytes
YTHDF1 Antibody Western Blot, validation (1:1000 dilution) in HaCaT/human keratinocytes (Cat no:17479-1-AP)