Tested Applications
| Positive WB detected in | Jurkat cells, pig spleen tissue, pig thymus tissue |
| Positive IHC detected in | human lymphoma tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
| Positive IF/ICC detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60200-1-Ig targets ZAP70 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, pig samples.
| Tested Reactivity | human, mouse, pig |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7943 Product name: Recombinant human ZAP70 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 149-493 aa of BC039039 Sequence: STLTHPQRRIDTLNSDGYTPEPARITSPDKPRPMPMDTSVYESPYSDPEELKDKKLFLKRDNLLIADIELGCGNFGSVRQGVYRMRKKQIDVAIKVLKQGTEKADTEEMMREAQIMHQLDNPYIVRLIGVCQAEALMLVMEMAGGGPLHKFLVGKREEIPVSNVAELLHQVSMGMKYLEEKNFVHRDLAARNVLLVNRHYAKISDFGLSKALGADDSYYTARSAGKWPLKWYAPECINFRKFSSRSDVWSYGVTMWEALSYGQKPYKKMKGPEVMAFIEQGKRMECPPECPPELYALMSDCWIYKWEDRPDFLTVEQRMRACYYSLASKVEGPPGSTQKAEAACA Predict reactive species |
| Full Name | zeta-chain (TCR) associated protein kinase 70kDa |
| Calculated Molecular Weight | 70 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC039039 |
| Gene Symbol | ZAP70 |
| Gene ID (NCBI) | 7535 |
| ENSEMBL Gene ID | ENSG00000115085 |
| RRID | AB_10944567 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P43403 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Zeta-associated protein (ZAP)-70, a member of the Syk family of tyrosine kinases, plays an important role in T-cell receptor signaling, natural killer (NK) cell activation and early B-cell development. ZAP-70 is expressed in a subset of cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) with unmutated immunoglobulin heavy-chain variable region (IgVH) genes and is associated with poor clinical outcome.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ZAP70 antibody 60200-1-Ig | Download protocol |
| IHC protocol for ZAP70 antibody 60200-1-Ig | Download protocol |
| WB protocol for ZAP70 antibody 60200-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ZAP70 Monoclonal antibody (1A11G9), catalog number 60200-1-Ig, has 9 citations. These appear in journals including Cell Research, Nature Communications, Journal of Advanced Research, Journal of Pharmaceutical Analysis, Frontiers in Pharmacology, Journal of Immunology, Cancer Research Communications, Cell Immunology, and Angiogenesis. Research fields span NK-cell antitumor immunity, T-cell activation and TCR signaling, renal inflammation, immune checkpoint targeting, anti-inflammatory mechanisms, cancer-associated fibroblast biology, T-cell differentiation, and bladder cancer metastasis.
| Species | Application | Title |
|---|---|---|
Cell Res CD55 organizes lipid raft-LCK signaling to potentiate NK-cell antitumor immunity | ||
Nat Commun Cell volume controlled by LRRC8A-formed volume-regulated anion channels fine-tunes T cell activation and function | ||
J Adv Res Podocyte-derived exosomes instruct dendritic cell-dependent CD8(+) T cell activation and proliferation in renal inflammation. | ||
J Pharm Anal A novel proteolysis-targeting chimera strategy targeting multiple immune checkpoints containing ITIMs enhances antitumor immunity. | ||
Front Pharmacol Study on the anti-inflammatory effects and mechanisms of gentisic acid based on the LPS-induced RAW264.7 cell inflammation model and the oxazolone-induced zebrafish inflammation model. | ||
Cancer Res Commun Collagen-Bearing Exosomes from Breast Cancer-Associated Fibroblasts Promote T-cell Dysfunction. |
