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ZAP70 Monoclonal antibody

ZAP70 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, pig

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1A11G9

Cat no : 60200-1-Ig

Synonyms

70 kDa zeta associated protein, SRK, STD, Syk related tyrosine kinase, Tyrosine protein kinase ZAP 70, TZK, ZAP 70, ZAP70



Tested Applications

Positive WB detected inJurkat cells, pig spleen tissue, pig thymus tissue
Positive IHC detected inhuman lymphoma tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 60200-1-Ig is ZAP70 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ZAP70 fusion protein Ag7943
Full Name zeta-chain (TCR) associated protein kinase 70kDa
Calculated molecular weight 70 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC039039
Gene symbol ZAP70
Gene ID (NCBI) 7535
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Zeta-associated protein (ZAP)-70, a member of the Syk family of tyrosine kinases, plays an important role in T-cell receptor signaling, natural killer (NK) cell activation and early B-cell development. ZAP-70 is expressed in a subset of cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) with unmutated immunoglobulin heavy-chain variable region (IgVH) genes and is associated with poor clinical outcome.

Protocols

Product Specific Protocols
WB protocol for ZAP70 antibody 60200-1-IgDownload protocol
IHC protocol for ZAP70 antibody 60200-1-IgDownload protocol
IF protocol for ZAP70 antibody 60200-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols