ZAP70 Monoclonal antibody, PBS Only (Detector)

• 

  WB analysis of Jurkat using 60200-1-Ig (same clone as 60200-1-PBS)

  WB result of ZAP70 antibody (60200-1-Ig, 1:10000) with si-control and si-ZAP70 transfected Jurkat cells.. This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 60200-1-Ig (same clone as 60200-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60200-1-Ig (ZAP70 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig spleen using 60200-1-Ig (same clone as 60200-1-PBS)

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 60200-1-Ig (ZAP70 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig thymus using 60200-1-Ig (same clone as 60200-1-PBS)

  pig thymus tissue were subjected to SDS PAGE followed by western blot with 60200-1-Ig (ZAP70 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 60200-1-Ig (same clone as 60200-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60200-1-Ig (ZAP70 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 60200-1-Ig (same clone as 60200-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60200-1-Ig (ZAP70 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 60200-1-Ig (same clone as 60200-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60200-1-Ig (ZAP70 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60200-1-Ig (same clone as 60200-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60200-1-Ig (ZAP70 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60200-1-Ig (same clone as 60200-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60200-1-Ig (ZAP70 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60200-1-Ig (same clone as 60200-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60200-1-Ig (ZAP70 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 60200-1-Ig (same clone as 60200-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60200-1-Ig (ZAP70 antibody), at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP51134-1

  Cytometric bead array standard curve of MP51134-1, ZAP70 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60200-3-PBS. Detection antibody: 60200-1-PBS. Standard:Ag7943. Range: 0.391-100 ng/mL.

• 

  Affinity and Kinetic Characterization of 60200-1-Ig

  Biolayer interferometry (BLl) kinetic assay of 60200-1-Ig against Human ZAP70 was performed. The affinity constant is 1.38 nM.

• 

  Affinity and Kinetic Characterization of 60200-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 60200-1-Ig against Human ZAP70 was performed. The affinity constant is 1.38 nM..

• Featured Product
• KD/KO Validated

ZAP70 Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Cat No. 60200-1-PBS

Clone No.1A11G9

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, pig

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Synonyms

1A11G9, 70 kDa zeta associated protein, 70 kDa zeta-chain associated protein, EC:2.7.10.2, SRK

---

Product Information

60200-1-PBS targets ZAP70 as part of a matched antibody pair:

MP51134-1: 60200-3-PBS capture and 60200-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7943

  Product name: Recombinant human ZAP70 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 149-493 aa of BC039039

  Sequence: STLTHPQRRIDTLNSDGYTPEPARITSPDKPRPMPMDTSVYESPYSDPEELKDKKLFLKRDNLLIADIELGCGNFGSVRQGVYRMRKKQIDVAIKVLKQGTEKADTEEMMREAQIMHQLDNPYIVRLIGVCQAEALMLVMEMAGGGPLHKFLVGKREEIPVSNVAELLHQVSMGMKYLEEKNFVHRDLAARNVLLVNRHYAKISDFGLSKALGADDSYYTARSAGKWPLKWYAPECINFRKFSSRSDVWSYGVTMWEALSYGQKPYKKMKGPEVMAFIEQGKRMECPPECPPELYALMSDCWIYKWEDRPDFLTVEQRMRACYYSLASKVEGPPGSTQKAEAACA

• Full Name: zeta-chain (TCR) associated protein kinase 70kDa
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC039039
• Gene Symbol: ZAP70
• Gene ID (NCBI): 7535
• ENSEMBL Gene ID: ENSG00000115085
• RRID: AB_10944567
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P43403
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Zeta-associated protein (ZAP)-70, a member of the Syk family of tyrosine kinases, plays an important role in T-cell receptor signaling, natural killer (NK) cell activation and early B-cell development. ZAP-70 is expressed in a subset of cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) with unmutated immunoglobulin heavy-chain variable region (IgVH) genes and is associated with poor clinical outcome.