ZNF638 Polyclonal antibody

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  WB analysis using 31300-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31300-1-AP (ZNF638 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 31300-1-AP

  IP result of anti-ZNF638 (IP:31300-1-AP, 4ug; Detection:31300-1-AP 1:3000) with Jurkat cells lysate 1360 ug.

ZNF638 Polyclonal Antibody for WB, IP, ELISA

Cat No. 31300-1-AP

Reviews (3)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

CTCL associated antigen se33 1, NP220, Nuclear protein 220, ZFML, zinc finger protein 638

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Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells, HepG2 cells, Jurkat cells
• Positive IP detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31300-1-AP targets ZNF638 in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZNF638 fusion protein Ag35296
• Full Name: zinc finger protein 638
• Calculated Molecular Weight: 221kDa
• Observed Molecular Weight: 270-300 kDa
• GenBank Accession Number: NM_014497.4
• Gene Symbol: ZNF638
• Gene ID (NCBI): 27332
• RRID: AB_3669937
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q14966
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZNF638 (Zinc finger protein 638) is also named as NP220 and ZFML. ZNF638 is a newly discovered member of zinc finger protein family for adipocyte function in vitro. And ZNF638regulates triglyceride metabolism via ANGPTL8 in an estrogen dependent manner (PMID: 38211696). ZNF638 plays a role as a transcriptional coregulator of adipocyte differentiation via induction of PPARγ in cooperation with CCAAT/enhancer binding proteins (C/EBPs) (PMID: 25024404). ZNF638 is localized in nuclear bodies enriched with splicing factors (PMID: 25024404). The ZNF638 protein is modified and exhibits a molecular weight of ~300 kDa (PMID: 33040080)

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Aleksandr (Verified Customer) (07-29-2025)

Antibody recognise a band of the expected size with some other prominent, likely non-specific ones

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: RKO

ZNF638 Antibody Western Blot validation (1:2000 dilution) in RKO (Cat no:31300-1-AP)

FH

Tsimafei (Verified Customer) (08-07-2024)

Great antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: U2OS

ZNF638 Antibody Western Blot validation (1:3000 dilution) in U2OS (Cat no:31300-1-AP)

FH

Julia (Verified Customer) (08-07-2024)

Band below 250 kDa marker is likely the Znf639 (~221kDa), there is also strong signal between 100-50kDa. I used 30ug of mESC protein extract.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: mESC

ZNF638 Antibody Western Blot validation (1:5000 dilution) in mESC (Cat no:31300-1-AP)