ZO-1 Monoclonal antibody

ZO-1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, Canine and More (1)

Applications

WB, IHC, IF

Conjugate

Unconjugated

CloneNo.

1G4A1

Cat no : 66452-1-Ig

Synonyms

Tight junction protein 1, Tight junction protein ZO 1, TJP1, ZO 1, ZO1, ZO-1, Zona occludens protein 1, Zonula occludens protein 1



Tested Applications

Positive WB detected inHEK-293 cells, HUVEC cells, HepG2 cells, COLO320 cells
Positive IHC detected inhuman pancreas cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells, MDCK cells, human breast cancer tissue
Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:750-1:3000
Sample-dependent, check data in validation data gallery

Product Information

66452-1-Ig targets ZO-1 in WB, IHC, IF applications and shows reactivity with human, Canine samples.

Tested Reactivity human, Canine
Cited Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ZO-1 fusion protein Ag16454
Full Name tight junction protein 1 (zona occludens 1)
Calculated molecular weight 1748 aa, 195 kDa
Observed molecular weight 230 kDa
GenBank accession numberBC111712
Gene symbol TJP1
Gene ID (NCBI) 7082
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tight junction (or zonula occludens) form the continuous intercellular barrier between epithelial and endothelial cells, which is required to separate tissue spaces and regulate selective movement of solutes across the epithelium and endothelium (PMID: 20066090). ZO-1 (also known as TJP1) is a peripheral membrane phosphoprotein located on the cytoplasmic face and is expressed in tight junctions of both epithelial and endothelial cells (PMID: 3528172). It binds the transmembrane proteins occludin and the claudins linking them to cytoskeletal actin (PMID: 17418867). ZO-1 belongs to a family of multidomain proteins known as the membrane-associated guanylate kinase homologs (MAGUKs). It is a pivotal tight junction protein and may be involved in signalling mechanisms regulating cell proliferation and differentiation (PMID: 22782886).

Protocols

Product Specific Protocols
WB protocol for ZO-1 antibody 66452-1-IgDownload protocol
IHC protocol for ZO-1 antibody 66452-1-IgDownload protocol
IF protocol for ZO-1 antibody 66452-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIF

J Am Heart Assoc

Native Coronary Collateral Microcirculation Reserve in Rat Hearts.

Authors - Xiucheng Liu
humanIHC

Front Oncol

LDHA Promotes Oral Squamous Cell Carcinoma Progression Through Facilitating Glycolysis and Epithelial-Mesenchymal Transition.

Authors - Hongshi Cai
humanIF

J Exp Clin Cancer Res

Tumor cell-derived SPON2 promotes M2-polarized tumor-associated macrophage infiltration and cancer progression by activating PYK2 in CRC.

Authors - Chengmei Huang
mouseWB

J Toxicol Sci

Effects of intragastric administration of La2O3 nanoparticles on mouse testes.

Authors - Lu Yuan
ratIF

Cardiology

Coronary Collateral Microcirculation Reserve Becomes Vestigial with Aging.

Authors - Jiali Chen
mouseWB

Brain Res

Mice with pre-existing tumors are vulnerable to postoperative cognitive dysfunction.

Authors - Tong Wu