|Positive WB detected in||HEK-293 cells, HUVEC cells, HepG2 cells, COLO320 cells|
|Positive IHC detected in||human pancreas cancer tissue, human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MDCK cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:200-1:800|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66452-1-Ig targets ZO-1 in WB, IHC, IF,ELISA applications and shows reactivity with human, Canine samples.
|Tested Reactivity||human, Canine|
|Host / Isotype||Mouse / IgG1|
|Immunogen||ZO-1 fusion protein Ag16454|
|Full Name||tight junction protein 1 (zona occludens 1)|
|Calculated molecular weight||1748 aa, 195 kDa|
|Observed molecular weight||230 kDa|
|GenBank accession number||BC111712|
|Gene ID (NCBI)||7082|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Tight junction (or zonula occludens) form the continuous intercellular barrier between epithelial and endothelial cells, which is required to separate tissue spaces and regulate selective movement of solutes across the epithelium and endothelium (PMID: 20066090). ZO-1 (also known as TJP1) is a peripheral membrane phosphoprotein located on the cytoplasmic face and is expressed in tight junctions of both epithelial and endothelial cells (PMID: 3528172). It binds the transmembrane proteins occludin and the claudins linking them to cytoskeletal actin (PMID: 17418867). ZO-1 belongs to a family of multidomain proteins known as the membrane-associated guanylate kinase homologs (MAGUKs). It is a pivotal tight junction protein and may be involved in signalling mechanisms regulating cell proliferation and differentiation (PMID: 22782886).
J Am Heart Assoc
Native Coronary Collateral Microcirculation Reserve in Rat Hearts.
LDHA Promotes Oral Squamous Cell Carcinoma Progression Through Facilitating Glycolysis and Epithelial-Mesenchymal Transition.
J Toxicol Sci
Effects of intragastric administration of La2O3 nanoparticles on mouse testes.
Coronary Collateral Microcirculation Reserve Becomes Vestigial with Aging.
Mice with pre-existing tumors are vulnerable to postoperative cognitive dysfunction.
Exp Cell Res
Sorafenib derivatives-functionalized gold nanoparticles confer protection against tumor angiogenesis and proliferation via suppression of EGFR and VEGFR-2.