"Alpha Tubulin Antibodies" Comparison
View side-by-side comparison of Alpha Tubulin antibodies from other vendors to find the one that best suits your research needs.
Tested Applications
| Positive WB detected in | HEK-293 cells, A549 cells, HeLa cells, NIH/3T3 cells, rat brain, C6 cells, mouse heart tissue, mouse liver tissue, rat liver tissue, rat heart tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue, human liver cancer tissue, human endometrial cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-Fro detected in | mouse testis tissue |
| Positive IF/ICC detected in | HeLa cells, HepG2 cells, MCF-7 cells, Lymphatic endothelial cells cells |
| Positive FC (Intra) detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11224-1-AP targets Alpha Tubulin in WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, CoIP, ELISA, Western Blot applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, canine, monkey, chicken, bovine, hamster, goat, bombyx mori |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1727 Product name: Recombinant human Tubulin-Alpha protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-451 aa of BC009314 Sequence: MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGVDSVEGEGEEEGEEY Predict reactive species |
| Full Name | tubulin, alpha 1b |
| Calculated Molecular Weight | 50 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC009314 |
| Gene Symbol | Alpha Tubulin |
| Gene ID (NCBI) | 10376 |
| RRID | AB_2210206 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P68363 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
What is the function of alpha tubulin?
Alpha-tubulin belongs to a large superfamily of tubulin proteins. There are a number of different subtypes that have a molecular weight of ~50kDa and are able to bind to beta-tubulin, forming a heterodimer that polymerises to microtubules as part of the cytoskeleton. These maintain cell structure, provide platforms for intracellular transport and are also involved in cell division.
Where is alpha-tubulin expressed?
Alpha tubulin is highly conserved and is present in nearly all eukaryotic cells as one of the building blocks of microtubules. The ubiquitous nature of this protein has led to its common use as a control protein for many tissue types as well as highlighting the structure of the cytoskeleton.
What are the post-translational modifications of alpha tubulin?
The function and properties of microtubules are drastically affected by the post-translational modifications undergone by tubulin, which may occur to the tubulin dimer directly or to the polymerised mictotubule. For example, the first modification to be identified was detyrosination1, as most alpha-tubulins have a tyrosine at their terminus. This process affects microtubules more than dimers and leads to patches of detyronisation along the structure, regulating protein interactions and allowing subcellular compartments to be defined.2,3 Polyglutamylation also occurs on several sites within the carboxy-terminal tails. However, to date, the most-studied alpha tubulin modification is related to acetylation of lysine 40 (K40).
1. Gundersen, G. G., Khawaja, S. & Bulinski, J. C. Postpolymerization detyrosination of alpha-tubulin: a mechanism for subcellular differentiation of microtubules. J. Cell Biol. 105, 251-64 (1987).
2. Galjart, N. Plus-End-Tracking Proteins and Their Interactions at Microtubule Ends. Curr. Biol. 20, R528-R537 (2010).
3. Jiang, K. & Akhmanova, A. Microtubule tip-interacting proteins: a view from both ends. Curr. Opin. Cell Biol. 23, 94-101 (2011).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Alpha Tubulin antibody 11224-1-AP | Download protocol |
| IF protocol for Alpha Tubulin antibody 11224-1-AP | Download protocol |
| IHC protocol for Alpha Tubulin antibody 11224-1-AP | Download protocol |
| IP protocol for Alpha Tubulin antibody 11224-1-AP | Download protocol |
| WB protocol for Alpha Tubulin antibody 11224-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Alpha Tubulin Polyclonal antibody (Cat# 11224-1-AP) has 2,439 total citations. Citations appear in high-impact journals including Nature, Science, Cancer Cell, Cell Metabolism, Cell Research, Nature Communications, Molecular Cell, Autophagy, and Redox Biology. Research fields span oncology, cell metabolism, autophagy, neurology, immunology, cardiology, microbiology, and general cell biology, with Western blot being the predominant application.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Targeting polarized phenotype of microglia via IL6/JAK2/STAT3 signaling to reduce NSCLC brain metastasis. | ||
Signal Transduct Target Ther Seryl tRNA synthetase cooperates with POT1 to regulate telomere length and cellular senescence. | ||
Signal Transduct Target Ther Targeting HECTD3-IKKα axis inhibits inflammation-related metastasis | ||
Signal Transduct Target Ther SARS-CoV-2 triggers inflammatory responses and cell death through caspase-8 activation. | ||
Signal Transduct Target Ther Phosphorylation determines the glucose metabolism reprogramming and tumor-promoting activity of sine oculis homeobox 1 | ||
Signal Transduct Target Ther Aberrant epithelial cell interaction promotes esophageal squamous-cell carcinoma development and progression |
Reviews
What customers say
Users consistently rate this antibody highly, praising its performance as a reliable loading control for Western Blot. Reviewers report clear, specific, and sharp bands with minimal background across a wide dilution range (1:1000–1:10000). Results are described as reproducible across mouse tissues, human cell lines, and primary cells. Several users note it outperforms GAPDH for quantification in certain contexts. It also performs well for immunohistochemistry and immunofluorescence, with strong and clean staining. Overall, reviewers recommend it as a dependable housekeeping antibody.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Bruna (Verified Customer) (06-10-2026) | It works very well for Western Blot, dilution 1:1000 or 1:2000, in both milk or BSA. The band is very clear and specific, and the results are reproducible. The signal is quite clean, with no non-specific background binding.
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Charlotte (Verified Customer) (04-07-2026) | We used this antibody in standard conditions : 5% milk TBST, overnight incubation
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Will (Verified Customer) (02-27-2026) | Works great for blots, very reproducible.
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Corre (Verified Customer) (09-19-2025) | Very clear and specific band
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Harvey (Verified Customer) (09-10-2025) | Housekeeper for mouse tissue western blot
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Patrick (Verified Customer) (07-16-2025) | Used for a flotation gradient and worked fine as a cytosolic marker. Even though we needed a longer exposure and incubation with ECL compared to actin.
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Samantha (Verified Customer) (05-10-2024) | Bands were faint but still visible when changes in contrast were applied. Some samples had big bands due to the large amounts present in the samples
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Kamal (Verified Customer) (02-15-2024) | One of the best antibody to detect alpha-tubulin. Works well when diluted in 1X TBST.
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Brogan (Verified Customer) (11-09-2022) | Used as a control for C elegans western blot
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WEI (Verified Customer) (03-03-2022) | Good antibody for detect tubulin proteins
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Maria (Verified Customer) (06-19-2021) | a Tubulin in human primary fibroblasts. 10 ug of total protein with DTT. 4-15 TGX gel. 3% BSA in PBST 1h RT. 1:1000 primary ab in BSA 3% in PBST (0.1% Tween) O/N 4ºC. Secondary ab 1:5000 1h RT.
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Azita (Verified Customer) (06-17-2021) | Immunocytochemistry labelling of (4% PFA) fixed SH-SY5Y cells by Alpha Tubulin Polyclonal antibody at dilution of 1:100 showed strong labelling.
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Chao (Verified Customer) (03-12-2020) | Good sharp clear band in western blot.
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Wei (Verified Customer) (01-30-2020) | Very good, sharp band for internal control.The protein is better than GAPDH for quantification in heart diseases.
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KARTHIKEYAN (Verified Customer) (11-20-2019) | I used this antibody in mouse brain endothelial cell line, it worked really well, i would definitely recommend this product.
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Pooja (Verified Customer) (11-05-2019) | This antibody works very well as loading control. Even lower dilution can be use.
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Yuan (Verified Customer) (11-02-2019) | Very nice staining for A549 cells with 1:200 dilution
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Kishor (Verified Customer) (01-30-2019) | I got good results for Western Blotting (1:1000) and IF (1:300)
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kk (Verified Customer) (11-21-2018) | Excellent product, single and sharp band in all my blots
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