|Positive WB detected in||mouse brain tissue, rat brain tissue|
|Positive IHC detected in||mouse cerebellum tissue, mouse brain tissue, rat brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Sample-dependent, check data in validation data gallery|
66412-1-Ig targets Alpha Synuclein in WB, IHC,ELISA applications and shows reactivity with human, rat, mouse samples.
|Tested Reactivity||human, rat, mouse|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Alpha Synuclein fusion protein Ag1285|
|Full Name||synuclein, alpha (non A4 component of amyloid precursor)|
|Calculated molecular weight||14 kDa|
|Observed molecular weight||18-19 kDa|
|GenBank accession number||BC013293|
|Gene ID (NCBI)||6622|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Alpha Synuclein (α-syn) is a 14-19 kDa phosphoprotein that is primarily localize to the presynaptic terminals of mature neurons, where it is involved in synaptic function and plasticity. α-syn has drawn intense interest ever since the late 1990s, when the first α-synuclein missense mutation was identified as a cause of familial Parkinson's disease (PD). Aggregated and hyper-phosphorylated forms of α-syn protein are the pathological hallmark of Lewy body disease, which includes Parkinson's disease (PD), diffuse Lewy body disease (DLBD), and Lewy body variant of Alzheimer's disease (LBV). This antibody can recognize all the isoforms of α-syn.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Sheela (Verified Customer) (07-16-2019)
This product worked as expected