TGFBI/BIGH3 Recombinant monoclonal antibody, PBS Only (Capture)

• 

  WB analysis of mouse eye using 80805-3-RR (same clone as 80805-3-PBS)

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 80805-3-RR (beta IG-H3/TGFBI antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80805-3-PBS in a different storage buffer formulation.

• 

  WB analysis of rat eye using 80805-3-RR (same clone as 80805-3-PBS)

  rat eye tissue were subjected to SDS PAGE followed by western blot with 80805-3-RR (beta IG-H3/TGFBI antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80805-3-PBS in a different storage buffer formulation.

• 

  WB analysis using 80805-3-RR (same clone as 80805-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80805-3-RR (beta IG-H3/TGFBI antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80805-3-PBS in a different storage buffer formulation.

• 

  Cytometric bead array sample test of MP02279-1

  The mean TGFBI/BIGH3 concentration was determined to be 3586.8 ng/mL in human serum.

• 

  Cytometric bead array standard curve of MP02279-1

  Cytometric bead array standard curve of MP02279-1, TGFBI/BIGH3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 80805-3-PBS. Detection antibody: 80805-2-PBS. Standard: Eg1233. Range: 0.781-100 ng/mL.

• 

  Affinity and Kinetic Characterization of 80805-3-RR

  Biolayer interferometry (BLl) kinetic assays of 80805-3-RR against Human TGFBI/BIGH3 were performed. The affinity constant is 2.62 nM.

TGFBI/BIGH3 Uni-rAb^® Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Cat No. 80805-3-PBS

Clone No.243108H4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

TGFBI, TGFBI / BIGH3, Beta ig h3, Beta ig-h3, BIGH3

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Product Information

80805-3-PBS targets TGFBI/BIGH3 as part of a matched antibody pair:

MP02279-1: 80805-3-PBS capture and 80805-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg1233

  Product name: recombinant human beta IG-H3/TGFBI protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 24-683 aa of NM_000358.3

  Sequence: GPAKSPYQLVLQHSRLRGRQHGPNVCAVQKVIGTNRKYFTNCKQWYQRKICGKSTVISYECCPGYEKVPGEKGCPAALPLSNLYETLGVVGSTTTQLYTDRTEKLRPEMEGPGSFTIFAPSNEAWASLPAEVLDSLVSNVNIELLNALRYHMVGRRVLTDELKHGMTLTSMYQNSNIQIHHYPNGIVTVNCARLLKADHHATNGVVHLIDKVISTITNNIQQIIEIEDTFETLRAAVAASGLNTMLEGNGQYTLLAPTNEAFEKIPSETLNRILGDPEALRDLLNNHILKSAMCAEAIVAGLSVETLEGTTLEVGCSGDMLTINGKAIISNKDILATNGVIHYIDELLIPDSAKTLFELAAESDVSTAIDLFRQAGLGNHLSGSERLTLLAPLNSVFKDGTPPIDAHTRNLLRNHIIKDQLASKYLYHGQTLETLGGKKLRVFVYRNSLCIENSCIAAHDKRGRYGTLFTMDRVLTPPMGTVMDVLKGDNRFSMLVAAIQSAGLTETLNREGVYTVFAPTNEAFRALPPRERSRLLGDAKELANILKYHIGDEILVSGGIGALVRLKSLQGDKLEVSLKNNVVSVNKEPVAEPDIMATNGVVHVITNVLQPPANRPQERGDELADSALEIFKQASAFSRASQRSVRLAPVYQKLLERMKH

• Full Name: transforming growth factor, beta-induced, 68kDa
• Calculated Molecular Weight: 75kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: NM_000358.3
• Gene Symbol: TGFBI
• Gene ID (NCBI): 7045
• ENSEMBL Gene ID: ENSG00000120708
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q15582
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TGFBI, also named as BIGH3, Kerato-epithelin and RGD-CAP, binds to type I, II, and IV collagens. TGFBI is an adhesion protein which may play an important role in cell-collagen interactions. In cartilage, it may be involved in endochondral bone formation. TGFBI is an extracellular matrix adaptor protein, it has been reported to be differentially expressed in transformed tissues. TGFBI is a predictive factor of the response to chemotherapy, and suggest the use of TGFBI-derived peptides as possible therapeutic adjuvants for the enhancement of responses to chemotherapy.(PMID:20509890) Defects in TGFBI are the cause of epithelial basement membrane corneal dystrophy (EBMD). Defects in TGFBI are the cause of corneal dystrophy Groenouw type 1 (CDGG1). Defects in TGFBI are the cause of corneal dystrophy lattice type 1 (CDL1). Defects in TGFBI are a cause of corneal dystrophy Thiel-Behnke type (CDTB). Defects in TGFBI are the cause of Reis-Buecklers corneal dystrophy (CDRB). Defects in TGFBI are the cause of lattice corneal dystrophy type 3A (CDL3A). Defects in TGFBI are the cause of Avellino corneal dystrophy (ACD).