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Recombinant Human FCGR3A/CD16a (F176V) protein (Myc Tag, His Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

Myc Tag, His Tag

Activity

EC50: 0.5-2 μg/mL

Cat no : Eg31662

Validation Data Gallery

  • Purity of Recombinant Human FCGR3A (F176V) was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Human FCGR3A (F176V) was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

  • Immobilized Human FCGR3A (F176V) (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Human IgG1 with a linear range of 0.5-2 μg/mL.

    Immobilized Human FCGR3A (F176V) (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Human IgG1 with a linear range of 0.5-2 μg/mL.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Immobilized Human FCGR3A (F176V) (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Human IgG1 with a linear range of 0.5-2 μg/mL.
Expression HEK293-derived Human FCGR3A protein Gly17-Gln208 (Accession# P08637, F176V) with a Myc tag and a His tag at the C-terminus.
GeneID 2214
Accession P08637
PredictedSize 24.3 kDa
SDS-PAGE 38-60 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD16, also known as the low affinity Fc gamma receptor III for IgG (FcγRIII), exists in two isoforms, FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B. CD16a is expressed on NK cells, macrophages, and placental trophoblasts as a polypeptide-anchored transmembrane protein while CD16b is expressed on neutrophils in a glycosylphosphatidylinositol (GPI)-anchored form. CD16a forms a heteromeric structure with the Fc epsilon RI (gamma) and/or CD3 (zeta) subunits. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.

References:

1. S Nagarajan, et al. (1995) J Biol Chem. 270(43):25762-70.
2. J E Gessner, et al. (1995) J Biol Chem. 270(3):1350-61.