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Recombinant Human CEACAM-8 protein (Myc Tag, His Tag)

Species

Human

Purity

>95 %, SDS-PAGE

Tag

Myc Tag, His Tag

Activity

EC50: 75-300 ng/mL

Cat no : Eg32133

Validation Data Gallery

  • Purity of Recombinant Human CEACAM-8 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human CEACAM-8 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Immobilized Human CEACAM-8 (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CEACAM-6 (Myc tag, His tag) with a linear range of 75-300 ng/mL.

    Immobilized Human CEACAM-8 (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CEACAM-6 (Myc tag, His tag) with a linear range of 75-300 ng/mL.

Product Information

Purity >95 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Immobilized Human CEACAM-8 (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CEACAM-6 (Myc tag, His tag) with a linear range of 75-300 ng/mL.
Expression HEK293-derived Human CEACAM-8 protein Gln35-Ser319 (Accession# P31997) with a Myc tag and a His tag at the C-terminus.
GeneID 1088
Accession P31997
PredictedSize 36.4 kDa
SDS-PAGE 55-70 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

A glycosylphosphatidyl-inositol-(GPI)-anchored glycoprotein, carcinoembryonic antigen (CEA)-related cell adhesion molecule 8 (CEACAM8), also known as CD66b, NCA-95 and CD67, was identified in human neutrophils. It is solely expressed by granulocytes, of which 95% are polymorphonuclear neutrophils (PMN). CEACAM8 is cell-adhesion proteins on neutrophils that belong to the human carcinoembryonic antigen (CEA) family. CEA family members are believed to function as intercellular adhesion molecules in vitro. CEACAM8 has been shown to adhere to the closely related molecule CEACAM6 (NCA-50/90 and CD66c), suggesting a role in the interaction between granulocytes or between granulocytes and epithelial cells expressing CEACAM6 in vivo.

References:

1.F Buchegger. et al. (1984). Int J Cancer. 33(5):643-649.
2.Linshu Zhao. et al. (2004). Br J Haematol. 125(5):666-673.
3.M Kuroki. et al. (2001). J Leukoc Biol. 70(4):543-550.
4.S Oikawa. et al. (1991). J Biol Chem. 266(13):7995-8001.
5.T Yamanka. et al. (1996). Biochem Biophys Res Commun.219(3):842-847.