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Recombinant Human Transferrin R/CD71 protein (His Tag)

Species

Human

Purity

>95 %, SDS-PAGE

Tag

His Tag

Activity

EC50: 54-216 ng/mL

Cat no : Eg0339

Validation Data Gallery

  • Purity of Recombinant Human Transferrin R was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human Transferrin R was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Immobilized Human Transferrin R (His tag) at 0.5 μg/mL (100 μL/well) can bind Biotinylated Human Transferrin (Myc tag, His tag) with a linear range of 54-216 ng/mL.

    Immobilized Human Transferrin R (His tag) at 0.5 μg/mL (100 μL/well) can bind Biotinylated Human Transferrin (Myc tag, His tag) with a linear range of 54-216 ng/mL.

Product Information

Purity >95 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Immobilized Human Transferrin R (His tag) at 0.5 μg/mL (100 μL/well) can bind Biotinylated Human Transferrin (Myc tag, His tag) with a linear range of 54-216 ng/mL.
Expression HEK293-derived Human Transferrin R protein Cys89-Phe760 (Accession# CAA25527) with a His tag at the N-terminus.
GeneID 7037
Accession CAA25527
PredictedSize 76 kDa
SDS-PAGE 75-90 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD71 also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is almost ubiquitously expressed, with the highest expression levels on some cells and tissues, including immature erythroid cells, placental tissue, and rapidly dividing cells. CD71 is involved in iron (Fe3+) uptake, and expression is regulated by the metabolic demand for iron. CD71 is present in actively proliferating cells and is essential for iron transport into proliferating cells.

References:

1. Speeckaert MM. et al. (2010). Crit Rev Clin Lab Sci. 47(5-6):213-228.
2. Dong HY. et al. (2011). Am J Surg Pathol. 35(5):723-732.
3. Jabara HH. et al. (2016). Nat Genet. 48(1):74-78.
4. Senyilmaz D. et al. (2015). Nature. 525(7567):124-128.