• Featured Product
  • KD/KO Validated

p38 MAPK Polyclonal antibody

p38 MAPK Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14064-1-AP

Synonyms

CSAID binding protein, CSBP, CSBP1, CSBP2, CSPB1, EXIP, MAP kinase 14, MAP kinase MXI2, MAP kinase p38 alpha, MAPK 14, MAPK14, MAX interacting protein 2, Mxi2, p38, p38ALPHA, PRKM14, PRKM15, RK, SAPK2A



Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, mouse heart tissue, rat heart tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14064-1-AP targets p38 MAPK in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity Artemisia sphaerocephala Krasch, CHICKEN, fish, human, mouse, pig, rabbit, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen p38 MAPK fusion protein Ag5115
Full Name mitogen-activated protein kinase 14
Calculated molecular weight 360 aa, 41 kDa
Observed molecular weight 35 kDa, 41 kDa
GenBank accession numberBC031574
Gene symbol MAPK14
Gene ID (NCBI) 1432
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAPK14(mitogen-activated protein kinase 14) is also named as SAPK2A, p38MAPK, CSBP1, RK, p38, EXIP, Mxi2, CSBP2, PRKM14, PRKM15, CSPB1, p38ALPHA and belongs to the MAP kinase subfamily. MAPK14-signaling is a central pathway for the integration of instructive signals in dendritic cells for T(H)17 differentiation and inflammation(PMID:22231518). It plays an important role in the regulation of hematopoietic stem cellself-renewal in vitro and inhibition of MAPK14 activation with a small molecule inhibitor may represent a novel approach to promote ex vivo expansion of hematopoietic stem cell(PMID:21198398). This protein has some isoforms with MW 29-31 kDa, 35 kDa and 41 kDa.

Protocols

Product Specific Protocols
WB protocol for p38 MAPK antibody 14064-1-APDownload protocol
IHC protocol for p38 MAPK antibody 14064-1-APDownload protocol
IF protocol for p38 MAPK antibody 14064-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Immunol

Activation of the Omega-3 Fatty Acid Receptor GPR120 Protects against Focal Cerebral Ischemic Injury by Preventing Inflammation and Apoptosis in Mice.

Authors - Zhiping Ren
ratWB

Stem Cell Res Ther

Catalpol promotes the osteogenic differentiation of bone marrow mesenchymal stem cells via the Wnt/β-catenin pathway.

Authors - Yu Zhu
ratWB

Front Pharmacol

SIRT6 Suppresses NFATc4 Expression and Activation in Cardiomyocyte Hypertrophy.

Authors - Zhenzhen Li
mouseWB

Int Immunopharmacol

Quercetin reduces Streptococcus suis virulence by inhibiting suilysin activity and inflammation.

Authors - Gen Li
mouseWB

J Exp Clin Cancer Res

Tobacco smoke induced hepatic cancer stem cell-like properties through IL-33/p38 pathway.

Authors - Chunfeng Xie
humanWB

Am J Physiol Heart Circ Physiol

NRP1 regulates HMGB1 in vascular endothelial cells under high homocysteine condition.

Authors - Yeshuo Ma