p38 MAPK Polyclonal antibody

• Featured Product
• KD/KO Validated

p38 MAPK Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

210

Cat no : 14064-1-AP

Synonyms

CSAID binding protein, CSBP, CSBP1, CSBP2, CSPB1, EXIP, MAP kinase 14, MAP kinase MXI2, MAP kinase p38 alpha, MAPK 14, MAPK14, MAX interacting protein 2, Mxi2, p38, p38ALPHA, PRKM14, PRKM15, RK, SAPK2A

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Validation Data Gallery

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  WB analysis of Jurkat using 14064-1-AP

  WB result of p38 antibody (14064-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-p38 transfected Jurkat cells.

• 

  WB analysis using 14064-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14064-1-AP (p38 MAPK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14064-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14064-1-AP (p38 MAPK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 14064-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14064-1-AP (p38 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 14064-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 14064-1-AP (p38 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14064-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14064-1-AP (p38 MAPK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14064-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14064-1-AP (p38 MAPK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 14064-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14064-1-AP (p38 MAPK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14064-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14064-1-AP (p38 MAPK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 14064-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14064-1-AP (p38 MAPK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14064-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14064-1-AP (p38 MAPK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14064-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14064-1-AP (p38 MAPK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 14064-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 14064-1-AP (p38 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse heart tissue, Jurkat cells, rat heart tissue, RAW 264.7 cells, Neuro-2a cells, NIH/3T3 cells
• Positive IHC detected in: human colon cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 203 publications below
• IHC: See 4 publications below
• IF: See 5 publications below

Product Information

14064-1-AP targets p38 MAPK in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, CHICKEN, fish, pig, rabbit, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: p38 MAPK fusion protein Ag5115
• Full Name: mitogen-activated protein kinase 14
• Calculated molecular weight: 360 aa, 41 kDa
• Observed molecular weight: 38-42 kDa
• GenBank accession number: BC031574
• Gene symbol: MAPK14
• Gene ID (NCBI): 1432
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MAPK14(mitogen-activated protein kinase 14) is also named as SAPK2A, p38MAPK, CSBP1, RK, p38, EXIP, Mxi2, CSBP2, PRKM14, PRKM15, CSPB1, p38ALPHA and belongs to the MAP kinase subfamily. MAPK14-signaling is a central pathway for the integration of instructive signals in dendritic cells for T(H)17 differentiation and inflammation(PMID:22231518). It plays an important role in the regulation of hematopoietic stem cellself-renewal in vitro and inhibition of MAPK14 activation with a small molecule inhibitor may represent a novel approach to promote ex vivo expansion of hematopoietic stem cell(PMID:21198398). This protein has some isoforms with MW 29-31 kDa, 35 kDa and 41 kDa.

Publications

Species	Application	Title
human	WB	J Immunol; Activation of the Omega-3 Fatty Acid Receptor GPR120 Protects against Focal Cerebral Ischemic Injury by Preventing Inflammation and Apoptosis in Mice.; Authors - Zhiping Ren
rat	WB	Stem Cell Res Ther; Catalpol promotes the osteogenic differentiation of bone marrow mesenchymal stem cells via the Wnt/β-catenin pathway.; Authors - Yu Zhu
rat	WB	Front Pharmacol; SIRT6 Suppresses NFATc4 Expression and Activation in Cardiomyocyte Hypertrophy.; Authors - Zhenzhen Li
mouse	WB	Int Immunopharmacol; Quercetin reduces Streptococcus suis virulence by inhibiting suilysin activity and inflammation.; Authors - Gen Li
mouse	WB	J Exp Clin Cancer Res; Tobacco smoke induced hepatic cancer stem cell-like properties through IL-33/p38 pathway.; Authors - Chunfeng Xie
human	WB	Am J Physiol Heart Circ Physiol; NRP1 regulates HMGB1 in vascular endothelial cells under high homocysteine condition.; Authors - Yeshuo Ma

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