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p38 MAPK Antibody 55 Publications

Rabbit Polyclonal| Catalog number: 14064-1-AP

Featured Product KD/KO validated

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Con: 45 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

p38 MAPK Antibody WB Jurkat cells 14064-1-AP

WB result of p38 antibody (14064-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-p38 transfected Jurkat cells

p38 MAPK Antibody WB HeLa cells 14064-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 14064-1-AP (p38 MAPK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

p38 MAPK Antibody IF HepG2 cells 14064-1-AP

Immunofluorescent analysis of ( 4% PFA ) fixed HepG2 cells using 14064-1-AP(p38 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat, A. sphaerocephala Krasch, rabbit
Positive WB detected in:
HeLa cells, Jurkat cells, mouse heart tissue, rat heart tissue
Positive IF detected in:
HepG2 cells
Recommended dilution:
WB : 1:500-1:2000
IF : 1:20-1:200
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
p38 MAPK fusion protein Ag5115
Full name:
mitogen-activated protein kinase 14
Calculated molecular weight:
360aa,41 kDa
Observed molecular weight:
35 kDa,41 kDa
GenBank accession number:
BC031574
Gene ID (NCBI):
1432
Gene symbol
MAPK14
Synonyms
CSAID binding protein, CSBP, CSBP1, CSBP2, CSPB1, EXIP, MAP kinase 14, MAP kinase MXI2, MAP kinase p38 alpha, MAPK 14, MAPK14, MAX interacting protein 2, Mxi2, p38, p38ALPHA, PRKM14, PRKM15, RK, SAPK2A

Background

MAPK14(mitogen-activated protein kinase 14) is also named as SAPK2A, p38MAPK, CSBP1, RK, p38, EXIP, Mxi2, CSBP2, PRKM14, PRKM15, CSPB1, p38ALPHA and belongs to the MAP kinase subfamily. MAPK14-signaling is a central pathway for the integration of instructive signals in dendritic cells for T(H)17 differentiation and inflammation(PMID:22231518). It plays an important role in the regulation of hematopoietic stem cellself-renewal in vitro and inhibition of MAPK14 activation with a small molecule inhibitor may represent a novel approach to promote ex vivo expansion of hematopoietic stem cell(PMID:21198398). This protein has some isoforms with MW 29-31 kDa, 35 kDa and 41 kDa.


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