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- KD/KO Validated
p38 MAPK Polyclonal antibody
p38 MAPK Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (5)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
170
Cat no : 14064-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells, mouse heart tissue, rat heart tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 164 publications below |
| IHC | See 3 publications below |
| IF | See 3 publications below |
Product Information
14064-1-AP targets p38 MAPK in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | Artemisia sphaerocephala Krasch, CHICKEN, fish, human, mouse, pig, rabbit, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | p38 MAPK fusion protein Ag5115 |
| Full Name | mitogen-activated protein kinase 14 |
| Calculated molecular weight | 360 aa, 41 kDa |
| Observed molecular weight | 35 kDa, 41 kDa |
| GenBank accession number | BC031574 |
| Gene symbol | MAPK14 |
| Gene ID (NCBI) | 1432 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MAPK14(mitogen-activated protein kinase 14) is also named as SAPK2A, p38MAPK, CSBP1, RK, p38, EXIP, Mxi2, CSBP2, PRKM14, PRKM15, CSPB1, p38ALPHA and belongs to the MAP kinase subfamily. MAPK14-signaling is a central pathway for the integration of instructive signals in dendritic cells for T(H)17 differentiation and inflammation(PMID:22231518). It plays an important role in the regulation of hematopoietic stem cellself-renewal in vitro and inhibition of MAPK14 activation with a small molecule inhibitor may represent a novel approach to promote ex vivo expansion of hematopoietic stem cell(PMID:21198398). This protein has some isoforms with MW 29-31 kDa, 35 kDa and 41 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for p38 MAPK antibody 14064-1-AP | Download protocol |
| IHC protocol for p38 MAPK antibody 14064-1-AP | Download protocol |
| IF protocol for p38 MAPK antibody 14064-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Immunol Activation of the Omega-3 Fatty Acid Receptor GPR120 Protects against Focal Cerebral Ischemic Injury by Preventing Inflammation and Apoptosis in Mice. | ||
Stem Cell Res Ther Catalpol promotes the osteogenic differentiation of bone marrow mesenchymal stem cells via the Wnt/β-catenin pathway. | ||
Front Pharmacol SIRT6 Suppresses NFATc4 Expression and Activation in Cardiomyocyte Hypertrophy. | ||
Int Immunopharmacol Quercetin reduces Streptococcus suis virulence by inhibiting suilysin activity and inflammation. | ||
J Exp Clin Cancer Res Tobacco smoke induced hepatic cancer stem cell-like properties through IL-33/p38 pathway. | ||
Am J Physiol Heart Circ Physiol NRP1 regulates HMGB1 in vascular endothelial cells under high homocysteine condition. |
