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p38 MAPK Polyclonal antibody
p38 MAPK Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (5)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
210
Cat no : 14064-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, mouse heart tissue, Jurkat cells, rat heart tissue, RAW 264.7 cells, Neuro-2a cells, NIH/3T3 cells |
Positive IHC detected in | human colon cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 3 publications below |
WB | See 203 publications below |
IHC | See 4 publications below |
IF | See 5 publications below |
Product Information
14064-1-AP targets p38 MAPK in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, CHICKEN, fish, pig, rabbit, zebrafish |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | p38 MAPK fusion protein Ag5115 |
Full Name | mitogen-activated protein kinase 14 |
Calculated molecular weight | 360 aa, 41 kDa |
Observed molecular weight | 38-42 kDa |
GenBank accession number | BC031574 |
Gene symbol | MAPK14 |
Gene ID (NCBI) | 1432 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MAPK14(mitogen-activated protein kinase 14) is also named as SAPK2A, p38MAPK, CSBP1, RK, p38, EXIP, Mxi2, CSBP2, PRKM14, PRKM15, CSPB1, p38ALPHA and belongs to the MAP kinase subfamily. MAPK14-signaling is a central pathway for the integration of instructive signals in dendritic cells for T(H)17 differentiation and inflammation(PMID:22231518). It plays an important role in the regulation of hematopoietic stem cellself-renewal in vitro and inhibition of MAPK14 activation with a small molecule inhibitor may represent a novel approach to promote ex vivo expansion of hematopoietic stem cell(PMID:21198398). This protein has some isoforms with MW 29-31 kDa, 35 kDa and 41 kDa.
Protocols
Product Specific Protocols | |
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WB protocol for p38 MAPK antibody 14064-1-AP | Download protocol |
IHC protocol for p38 MAPK antibody 14064-1-AP | Download protocol |
IF protocol for p38 MAPK antibody 14064-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Immunol Activation of the Omega-3 Fatty Acid Receptor GPR120 Protects against Focal Cerebral Ischemic Injury by Preventing Inflammation and Apoptosis in Mice. | ||
Stem Cell Res Ther Catalpol promotes the osteogenic differentiation of bone marrow mesenchymal stem cells via the Wnt/β-catenin pathway. | ||
Front Pharmacol SIRT6 Suppresses NFATc4 Expression and Activation in Cardiomyocyte Hypertrophy. | ||
Int Immunopharmacol Quercetin reduces Streptococcus suis virulence by inhibiting suilysin activity and inflammation. | ||
J Exp Clin Cancer Res Tobacco smoke induced hepatic cancer stem cell-like properties through IL-33/p38 pathway. | ||
Am J Physiol Heart Circ Physiol NRP1 regulates HMGB1 in vascular endothelial cells under high homocysteine condition. |