|Positive WB detected in||HeLa cells, HEK-293 cells, pig heart tissue, human heart tissue, Jurkat cells, MCF-7 cells, HSC-T6 cells|
|Positive IHC detected in||human lung cancer tissue, human gliomas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:2000-1:6000|
|Immunohistochemistry (IHC)||IHC : 1:250-1:1000|
|Sample-dependent, check data in validation data gallery|
66234-1-Ig targets p38 MAPK in WB, IHC, ELISA applications and shows reactivity with human, pig samples.
|Tested Reactivity||human, pig|
|Cited Reactivity||human, mouse, pig, rat|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||p38 MAPK fusion protein Ag5797|
|Full Name||mitogen-activated protein kinase 14|
|Calculated molecular weight||360 aa, 41 kDa|
|Observed molecular weight||38-42 kDa|
|GenBank accession number||BC031574|
|Gene ID (NCBI)||1432|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
MAPK14(mitogen-activated protein kinase 14) is also named as SAPK2A, p38MAPK, CSBP1, RK, p38, EXIP, Mxi2, CSBP2, PRKM14, PRKM15, CSPB1, p38ALPHA and belongs to the MAP kinase subfamily. MAPK14-signaling is a central pathway for the integration of instructive signals in dendritic cells for T(H)17 differentiation and inflammation(PMID:22231518). It plays an important role in the regulation of hematopoietic stem cellself-renewal in vitro and inhibition of MAPK14 activation with a small molecule inhibitor may represent a novel approach to promote ex vivo expansion of hematopoietic stem cell(PMID:21198398). This protein has 4 isoforms produced by alternative splicing.
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