• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human HGF protein


5-25 ng/mL

Specific Activity

minimally 2.50 x 105 IU/mg

Species Reactivity




Cat no : HZ-1084


F TCF, Hepatocyte growth factor, Hepatopoeitin A, HGF, HGFB, HPTA, Scatter factor, SF

Technical Specifications

GeneID 3082
Species Human
Expression HEK293
Activity(Primary) 5-25 ng/mL
Specific Activityminimally 2.50 x 105 IU/mg
Purity >95%
Endotoxin<1 EU/µg
Accession NumberP14210
Molecular Mass 85 kDa reduced, 70 kDa non-reduced, single chain, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,monkey,mouse,canine,rat,zebrafish

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.


Hepatocyte growth factor (HGF) is the most potent mitogen of mature hepatocytes in primary culture. It is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin (PMID 1530787, 2531289). HGF exerts a variety of effects on several cells, such as inflammation, tissue repair, morphogenesis, angiogenesis, tumour propagation, immunomodulation of viral infections and cardio-metabolic activities (PMID: 1846706; 15070743; 1383237; 1827664).


F TCF, Hepatocyte growth factor, Hepatopoeitin A, HGF, HGFB, HPTA, Scatter factor, SF



J Cell Biol

Isotropic actomyosin dynamics promote organization of the apical cell cortex in epithelial cells.

Authors - ChristophKlingner

Pharmacol Res

The natural product berberine synergizes with osimertinib preferentially against MET-amplified osimertinib-resistant lung cancer via direct MET inhibition

Authors - Zhen Chen

Toxicol Sci

In Silico Modeling for the Prediction of Dose and Pathway-Related Adverse Effects in Humans From In Vitro Repeated-Dose Studies.

Authors - SebastianKlein

Toxicol Lett

Tools for studying the metabolism of new psychoactive substances for toxicological screening purposes - A comparative study using pooled human liver S9, HepaRG cells, and zebrafish larvae.

Authors - Lilian H J Richter

Anal Bioanal Chem

Metabolic fate of desomorphine elucidated using rat urine, pooled human liver preparations, and human hepatocyte cultures as well as its detectability using standard urine screening approaches.

Authors - Lilian H JRichter

J Pharm Biomed Anal

Pooled human liver preparations, HepaRG, or HepG2 cell lines for metabolism studies of new psychoactive substances? A study using MDMA, MDBD, butylone, MDPPP, MDPV, MDPB, 5-MAPB, and 5-API as examples.

Authors - Lilian H JRichter


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Daman (Verified Customer) (09-11-2023)

We originally sourced our HGF from another company but they had poor availability, not only did this arrive quickly it was considerably cheaper and worked just as well.

  • Applications: Cell culture
  • Cell Tissue Type: Human endometrial organoids