• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IFN alpha 2A protein


Typically ≤ 0.06 ng/mL

Specific Activity

minimally 1.00 x 107 IU/mg

Species Reactivity




Cat no : HZ-1066


IFN alpha 2, IFN alpha 2A, IFNA, IFNA2, INFA2, Interferon alpha 2, Interferon alpha A, interferon, alpha 2, LeIF A

Technical Specifications

GeneID 3440
Species Human
Expression HEK293
Activity(Primary) Typically ≤ 0.06 ng/mL
Specific Activityminimally 1.00 x 107 IU/mg
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP01563
Molecular Mass 18 to 22 kDa reduced, 14 to 19 kDa non-reduced, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.


Interferon alpha 2 is a type 1 interferon. Interferon alpha 2 is secreted by virus-infected cells and inhibits other cells from further infection. Recombinant human interferon alpha 2 has been approved for therapeutic application in a range of human oncological and viral diseases. Three Interferon alpha 2 subvariants (2A, 2B, 2C) differ by only one or two amino acids at positions 23 and/or 34 of the mature protein (PMID: 25982860; 10672347; 1694761).


IFN alpha 2, IFN alpha 2A, IFNA, IFNA2, INFA2, Interferon alpha 2, Interferon alpha A, interferon, alpha 2, LeIF A



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Using an Old Drug to Target a New Drug Site: Application of Disulfiram to Target the Zn-Site in HCV NS5A Protein.

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Int J Mol Sci

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Cell Mol Immunol

BCR-ABL-specific CD4+ T-helper cells promote the priming of antigen-specific cytotoxic T cells via dendritic cells.

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ACS Infect Dis

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Cancer Sci

Combination of tumor necrosis factor-α and epidermal growth factor induces the adrenergic-to-mesenchymal transdifferentiation in SH-SY5Y neuroblastoma cells.

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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Christine (Verified Customer) (12-14-2021)

I induced the expression of interferon responsive genes by treating HEK293T cells for 24 or 48h with various concentrations of IFNalpha2a. Samples were then analysed by western blot for the detection of an IFN induced protein, which was absent at t=0, clearly induced at 24h and even better expressed at 48h. The lowest amount of IFN analysed here was 3 ng/ml. I also tried IFN beta (cat number HZ-1298) at the same time and the results were also very good, identical.

  • Applications: Cell culture
  • Cell Tissue Type: HEK293T