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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

Animal-free Recombinant Human LIF


Typically ≤ 0.2 ng/mL EC50

Species Reactivity




Cat no : HZ-1292


CDF, D factor, DIA, Emfilermin, HILDA, Leukemia inhibitory factor, LIF, Melanoma derived LPL inhibitor, MLPLI

Technical Specifications

GeneID 3976
Species Human
Expression HEK293
Activity Typically ≤ 0.2 ng/mL EC50
Purity >95%
Molecular Mass 35-53 kDa, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20oC) and (-80oC). Upon reconstitution we recommend that the solution can be stored at (4oC) for short term or at (-20oC) to (-80oC) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1x PBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Product Description

Recombinant Human LIF is a pleiotropic factor produced by numerous cell types which includes myelomonocytic lineages, T cells, fibroblasts, melanoma, liver, and heart. LIF promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Other activities include the stimulation of acute phase protein synthesis by hepatocytes, stimulation of differentiation of cholinergic nerves, and suppression of adipogenesis by inhibiting the lipoprotein lipase in adipocytes. Mature human LIF (180 aa) shares 78%, 82%, 91%, 88 and 87% aa sequence identity with mouse, rat, canine, bovine, and porcine LIF, respectively. To reduce spontaneous differentiation, LIF is typically added to stem cell culture medium.


LIF is a pleiotropic cytokine with a variety of roles. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface (PMID: 29620145; 29391217; 29271384)


CDF, D factor, DIA, Emfilermin, HILDA, Leukemia inhibitory factor, LIF, Melanoma derived LPL inhibitor, MLPLI