• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human LIF protein

Activity

Typically ≤ 1 ng/mL EC50 TF-1 Cell assay. 0.045-0.25 ng/mL EC50 in M1 cell assay

Specific Activity

9.5 x 10^6 IU/mg calibrated against LIF reference standard (NIBSC cat no: 93/562). Reference use only. Specific activity is not routinely tested

Species Reactivity

human

Purity

>95%

Cat no : HZ-1292

Synonyms

CDF, D factor, DIA, Emfilermin, HILDA, Leukemia inhibitory factor, LIF, Melanoma derived LPL inhibitor, MLPLI



Technical Specifications

GeneID 3976
Species Human
Expression HEK293
Activity Typically ≤ 1 ng/mL EC50 TF-1 Cell assay. 0.045-0.25 ng/mL EC50 in M1 cell assay
Specific Activity9.5 x 10^6 IU/mg calibrated against LIF reference standard (NIBSC cat no: 93/562). Reference use only. Specific activity is not routinely tested
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP15018
Molecular Mass 35-53 kDa, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

LIF is a pleiotropic cytokine with a variety of roles. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface (PMID: 29620145; 29391217; 29271384)

Synonyms

CDF, D factor, DIA, Emfilermin, HILDA, Leukemia inhibitory factor, LIF, Melanoma derived LPL inhibitor, MLPLI

Publications

SpeciesTitle
mouse

Nucleic Acids Res

Chromatin-contact atlas reveals disorder-mediated protein interactions and moonlighting chromatin-associated RBPs.

Authors - Mahmoud-Reza Rafiee

Reprod Biol Endocrinol

Expression of transcriptional factor EB (TFEB) in differentiating spermatogonia potentially promotes cell migration in mouse seminiferous epithelium.

Authors - Yue Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Irina (Verified Customer) (07-01-2021)

LIF cytokine was successfully used at 10ng/ml concentration for the culture of mES cells and their maintenance in a undifferentiated state

  • Applications: Cell culture
  • Cell Tissue Type: mESCs