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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

Animal-free Recombinant Human TNF alpha


Typically ≤ 0.5 ng/mL EC50

Species Reactivity




Cat no : HZ-1014


Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNFSF2, Tumor necrosis factor

Technical Specifications

GeneID 7124
Species human
Expression HEK293
Activity Typically ≤ 0.5 ng/mL EC50
Purity >95%
Molecular Mass 17 kDa, non-disulfide bonded homotrimer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human, mouse

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20oC) and (-80oC). Upon reconstitution we recommend that the solution can be stored at (4oC) for short term or at (-20oC) to (-80oC) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile
1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Product Description

Animal-free Recombinant Human TNF alpha is expressed in human 293 cells and has been shown by gel filtration to be predominantly a non-covalently linked homotrimer, with a molecular mass of 51 kDa. The E. coli form is not a trimer. The cytokine is produced in a human cell express ion system with serum free, chemically defined media.


Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNFSF2, Tumor necrosis factor



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