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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

Animal-free Recombinant Human TNF alpha

Activity

Typically ≤ 0.5 ng/mL EC50

Species Reactivity

human

Purity

>95%

Cat no : HZ-1014

Synonyms

Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNFSF2, Tumor necrosis factor



Technical Specifications

GeneID 7124
Species human
Expression HEK293
Activity Typically ≤ 0.5 ng/mL EC50
Purity >95%
Molecular Mass 17 kDa, non-disulfide bonded homotrimer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human, mouse

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20oC) and (-80oC). Upon reconstitution we recommend that the solution can be stored at (4oC) for short term or at (-20oC) to (-80oC) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile
1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Product Description

Animal-free Recombinant Human TNF alpha is expressed in human 293 cells and has been shown by gel filtration to be predominantly a non-covalently linked homotrimer, with a molecular mass of 51 kDa. The E. coli form is not a trimer. The cytokine is produced in a human cell express ion system with serum free, chemically defined media.

Synonyms

Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNFSF2, Tumor necrosis factor

Publications

SpeciesTitle
human

Am J Obstet Gynecol

Progesterone inhibits in vitro fetal membrane weakening.

Authors - Deepak Kumar
human

Acta Biochim Biophys Sin (Shanghai)

miR-101-3p induces vascular endothelial cell dysfunction by targeting tet methylcytosine dioxygenase 2.

Authors - Qiaoli Chen
human

Bioorg Med Chem Lett

One unique steroidal sapogenin obtained through the microbial transformation of ruscogenin by Phytophthora cactorum ATCC 32134 and its potential inhibitory effect on tissue factor (TF) procoagulant activity.

Authors - Nai-DongChen
human

Oncol Rep

Protein-bound polysaccharide-K augments the anticancer effect of fluoropyrimidine derivatives possibly by lowering dihydropyrimidine dehydrogenase expression in gastrointestinal cancers.

Authors - EijiMekata
human

PLoS One

Leukemia Mediated Endothelial Cell Activation Modulates Leukemia Cell Susceptibility to Chemotherapy through a Positive Feedback Loop Mechanism.

Authors - BaharehPezeshkian
human

Life Sci

Cynaropicrin is dual regulator for both degradation factors and synthesis factors in the cartilage metabolism.

Authors - TeruakiMasutani