• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human TNF alpha protein- GMP grade

EC50

0.002-0.026 ng/mL EC50

Specific Activity

1.27x10^7 IU/mg calibrated against TNF alpha reference standard (NIBSC cat no: 17/232). Reference use only. Specific activity is not routinely tested

Species Reactivity

human,mouse

Purity

>95%

ISO 13485 certified HumanKine® human cell-expressed cytokines and growth factors

Cat no : HZ-1014-GMP

Synonyms

Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNFSF2, Tumor necrosis factor



Technical Specifications

GeneID 7124
Species Human
Expression HEK293
EC50 0.002-0.026 ng/mL EC50
Specific Activity1.27x10^7 IU/mg calibrated against TNF alpha reference standard (NIBSC cat no: 17/232). Reference use only. Specific activity is not routinely tested
Purity >95%
Endotoxin<0.1 EU/µg
Accession NumberP01375
Molecular Mass 17 kDa, non-disulfide bonded homotrimer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane-bound protein and free soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced mostly by activated macrophages, CD4+ lymphocytes, NK cells, neutrophils, eosinophils, and neurons. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation (PMID: 20194223).

Synonyms

Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNFSF2, Tumor necrosis factor