- HEK293 expressed
- Endotoxin-free
- Animal-component free
HumanKine® recombinant human Wnt3A protein
Activity(Primary)
1-17 ng/mL EC50 in MC3T3 E1 cells.
Species Reactivity
human,mouse
Purity
>90%
Cat no : HZ-1296
Synonyms
Protein Wnt 3a, WNT3A
Validation Data Gallery
Technical Specifications
GeneID | 89780 |
Species | Human |
Expression | HEK293 |
Activity(Primary) | 1-17 ng/mL EC50 in MC3T3 E1 cells. |
Activity(Secondary) | 25-500 ng/mL EC50 in reporter cells |
Purity | >90% |
Endotoxin | <1 EU/μg |
Accession Number | P56704 |
Molecular Mass | 42 kDa |
Formulation | 1x PBS pH 7.4 + 1% CHAPS, See Certificate of Analysis for details |
Species Reactivity | human,mouse |
Stability and Reconstitution
Stability and Storage | Product Form | Temperature Conditions | Storage Time (From Date of Receipt) |
---|---|---|---|
Lyophilized | -20°C to -80°C | Until Expiry Date | |
Lyophilized | Room Temperature | 2 weeks | |
Reconstituted as per CofA | -20°C to -80°C | 6 months | |
Reconstituted as per CofA | 4°C | 1 week | |
Avoid repeated freeze-thaw cycles. |
Reconstitution | Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to ≤100μg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). |
Background
Wnt3A is a member of the Wnt family that consists of structurally linked genes that encode secreted signalling proteins. This protein is associated with oncogenesis and also regulation of cell fate and patterning during embryogenesis. It plays a key role in maintaining the integrity of embryonic and adult tissues. The post-translational glycosylation and acylation of Wnt are essential for their efficient secretion and biological functions (PMID: 8244403; 11834740)
Synonyms
Protein Wnt 3a, WNT3A
Publications
Species | Title |
---|---|
Elife Differentiation of mouse fetal lung alveolar progenitors in serum-free organotypic cultures. | |
Sci Signal Intercellular transmission of the unfolded protein response promotes survival and drug resistance in cancer cells. |