• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human Wnt3A protein


25-500 ng/mL in reporter cells

Species Reactivity




Cat no : HZ-1296


Protein Wnt 3a, WNT3A

Technical Specifications

GeneID 89780
Species Human
Expression HEK293
Activity(Primary) 25-500 ng/mL in reporter cells
Activity(Secondary)1-17 ng/mL in MC3T3 E1 cells.
Purity >90%
Endotoxin<1 EU/μg
Accession NumberP56704
Molecular Mass 42 kDa reduced, 40 kDa non-reduced, monomer, glycosylated
Formulation 1x PBS pH 7.4 + 1% CHAPS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.1 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.


Wnt3A is a member of the Wnt family that consists of structurally linked genes that encode secreted signalling proteins. This protein is associated with oncogenesis and also regulation of cell fate and patterning during embryogenesis. It plays a key role in maintaining the integrity of embryonic and adult tissues. The post-translational glycosylation and acylation of Wnt are essential for their efficient secretion and biological functions (PMID: 8244403; 11834740)


Protein Wnt 3a, WNT3A




Differentiation of mouse fetal lung alveolar progenitors in serum-free organotypic cultures.

Authors - Konstantinos Gkatzis

Sci Signal

Intercellular transmission of the unfolded protein response promotes survival and drug resistance in cancer cells.

Authors - Jeffrey J Rodvold

Immun Inflamm Dis

NR5A2 promotes malignancy progression and mediates the effect of cisplatin in cutaneous squamous cell carcinoma

Authors - Wang Ye