HumanKine® recombinant human Wnt3A protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

1-17 ng/mL EC50 in MC3T3 E1 cells.

Species Reactivity

human,mouse

Purity

>90%

Publications

2

Cat no : HZ-1296

Synonyms

Protein Wnt 3a, WNT3A

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Validation Data Gallery

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  Purity of recombinant human Wnt3A was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

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  Recombinant human Wnt3A (HZ-1296) induces dose-dependent luciferase production in a HEK293 TCF/LEF reporter cell line. Luciferase production was assessed by One-Step™ luciferase assay Kit. HEK293 TCF/LEF reporter cells were treated with increasing concentrations of recombinant Wnt3A for 6 hours. The EC50 was determined using a 4-Parameter non-linear regression model. The EC50 range is 25-125 ng/mL.

• 

  Recombinant human Wnt3A (HZ-1296) induces dose-dependent alkaline phosphatase production in MC3T3 E1 mouse preosteoblast cells using pNPP as chromogenic substrate. MC3T3 E1 cells were treated with increasing concentrations of recombinant Wnt3A for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 2-17 ng/mL.

• 

  Proteintech Wnt3A (HZ-1296) demonstrates a 3-fold lower EC50 and greater induction of luciferase production than a leading competitor. Recombinant human Wnt3A induces dose-dependent luciferase production in a HEK293 TCF/LEF reporter cell line. Luciferase production was assessed by One-Step™ luciferase assay Kit. HEK293 TCF/LEF reporter cells were treated with increasing concentrations of recombinant Wnt3A for 6 hours. The EC50 was determined using a 4-Parameter non-linear regression model. The EC50 range is 25-125 ng/mL.

• 

  Human induced pluripotent cells(iPSCs) were differentiated to definitive endoderm using HumanKine growth factors (Activin A- HZ-1138, bFGF-HZ-1285, BMP-4- HZ-1045, and Wnt3A-HZ-1296). The differentiation was confirmed by detection of expression of FOXA2(Red) and downregulation of pluripotency marker NANOG(yellow), in immunofluorescence

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Technical Specifications

• GeneID: 89780
• Species: Human
• Expression: HEK293
• Activity(Primary): 1-17 ng/mL EC50 in MC3T3 E1 cells.
• Activity(Secondary): 25-500 ng/mL EC50 in reporter cells
• Purity: >90%
• Endotoxin: <1 EU/μg
• Accession Number: P56704
• Molecular Mass: 42 kDa
• Formulation: 1x PBS pH 7.4 + 1% CHAPS, See Certificate of Analysis for details
• Species Reactivity: human,mouse

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to ≤100μg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

Wnt3A is a member of the Wnt family that consists of structurally linked genes that encode secreted signalling proteins. This protein is associated with oncogenesis and also regulation of cell fate and patterning during embryogenesis. It plays a key role in maintaining the integrity of embryonic and adult tissues. The post-translational glycosylation and acylation of Wnt are essential for their efficient secretion and biological functions (PMID: 8244403; 11834740)

Synonyms

Protein Wnt 3a, WNT3A

Publications

Species	Title
human	Elife; Differentiation of mouse fetal lung alveolar progenitors in serum-free organotypic cultures.; Authors - Konstantinos Gkatzis
human	Sci Signal; Intercellular transmission of the unfolded protein response promotes survival and drug resistance in cancer cells.; Authors - Jeffrey J Rodvold