1X10^6 human peripheral blood lymphocytes were surface stained with 0.5 ug Anti-Human CD45 (65064-1-Ig, Clone: F10-89-4 ) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1) at dilution 1:1000 (green), or 0.5 ug Mouse IgG2a Isotype Control (black). Cells were not fixed.