ubiquitin Recombinant antibody

ubiquitin Recombinant Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


Human, mouse, rat, hamster, Dog, Spinach, Yeast and More (1)







Cat no : 80992-1-RR


FLJ25987, Polyubiquitin B, UBB, ubiquitin, ubiquitin B

Tested Applications

Positive WB detected inNIH/3T3 cells, MG132 treated NIH/3T3 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:10000-1:100000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80992-1-RR targets ubiquitin in WB, IP, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat, hamster, Dog, Spinach, Yeast samples.

Tested Reactivity Human, mouse, rat, hamster, Dog, Spinach, Yeast
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ubiquitin fusion protein Ag0260
Full Name ubiquitin B
GenBank Accession NumberBC000379
Gene Symbol UBB
Gene ID (NCBI) 7314
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ubiquitin B (UBB) is a member of ubiquitin family, one of the most conserved proteins known. Ubiquitin B is required for ATP-dependent, non-lysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin B is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression.When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome. Interestingly ubiquitin also becomes covalently bonded to many types of pathological inclusions which appear to be resistant to normal degradation.


Product Specific Protocols
WB protocol for ubiquitin antibody 80992-1-RRDownload protocol
IHC protocol for ubiquitin antibody 80992-1-RRDownload protocol
IF protocol for ubiquitin antibody 80992-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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