1X10^6 C57BL/6 mouse splenocytes were surface stained with CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) and 0.5 ug FITC Anti-Mouse CD3ε (FITC-65060, Clone:145-2C11) or 0.5 ug FITC Armenian Hamster IgG Isotype Control (PIP). Cells were not fixed.

1X10^6 mouse splenocytes were surface stained with 0.1 ug APC Anti-Mouse CD3ε (APC-65060, Clone: 145-2C11) or 0.1 ug Control Antibody. Cells were not fixed.

1X10^6 C57BL/6 mouse splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) and 0.5 ug Anti-Mouse CD3ε (65060-1-Ig, Clone:145-2C11) or 0.5 ug Armenian Hamster IgG Isotype Control, and FITC anti-Armenian Hamster IgG antibody at dilution 1:100. Cells were not fixed.

Immunofluorescent analysis of mouse splenocytes using CD3ε antibody (65060-1-Ig, Clone: 145-2C11 ) at dilution of 1:500 and FITC Goat anti-Hamster(Armenian) IgG.

1X10^6 C57BL/6 mouse splenocytes were surface co-stained with PE Anti-Mouse CD4 (GK1.5) and 0.5 ug CoraLite® Plus 488 Anti-Mouse CD3ε (CL488-65060, Clone:145-2C11) or 0.5 ug Armenian Hamster IgG isotype Control. Cells were not fixed.

1X10^6 C57BL/6 mouse splenocytes were surface co-stained with 0.25 ug FITC Plus Anti-Mouse CD4 (GK1.5) (FITC-65104, Clone: GK1.5) and 0.5 ug CoraLite® Plus 647 Anti-Mouse CD3ε (CL647-65060, Clone:145-2C11) or 0.5 ug CoraLite® Plus 647 Armenian Hamster IgG Isotype Control (PIP) (CL647-65210, Clone: PIP). Cells were not fixed.

1X10^6 mouse splenocytes were surface co-stained with FITC Anti-Mouse CD4 and 0.5 ug CoraLite®594 Anti-Mouse CD3ε (CL594-65060, Clone: 145-2C11) or 0.5 ug Isotype Control. Cells were not fixed.

1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.10 ug PE Anti-Mouse CD3ε (PE-65060, Clone:145-2C11) (red) or 0.10 ug isotype control antibody (blue). Cells were not fixed.