1X10^6 mouse splenocytes were surface stained with APC Anti-Mouse CD4 (GK1.5) (APC-65104, Clone: GK1.5) and 1 ug FITC Plus Anti-Mouse CD3 (FITC-65077, Clone: 17A2) or 1 ug isotype control. Cells were not fixed.

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1X10^6 mouse splenocytes were surface co-stained with Anti-Mouse CD4 and 0.5 ug PE Anti-Mouse CD3 (PE-65077, Clone: 17A2) (red) or 0.5 ug Isotype Control. Cells were not fixed.

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1X10^6 mouse splenocytes were surface stained with 0.5 ug PE Anti-Mouse CD3 (PE-65077, Clone:17A2) (red), or 0.5 ug Isotype Control. Cells were not fixed.

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1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.5 ug APC-anti-mouse CD3 (APC-65077, clone 17A2) (red) or 0.5 ug APC-rat IgG2b isotype control (blue). Cells were not fixed.

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1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD3 (65077-1-Ig, Clone: 17A2) and FITC anti-Rat IgG2b Antibody at dilution 1:100, or 0.5 ug Rat IgG2b Isotype Control (LTF-2) (65211-1-Ig, Clone: LTF-2) and FITC anti-Rat IgG2b Antibody at dilution 1:100. Cells were co-stained with APC Anti-Mouse CD4 (GK1.5) (APC-65104, Clone: GK1.5). Cells were not fixed.

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1X10^6 mouse splenocytes were surface stained with 5 ul CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) (CL647-65104, Clone: GK1.5) and CoraLite® Plus 488-Rat IgG2b isotype control (left) or 5 ul CoraLite® Plus 488 Anti-Mouse CD3 (CL488-65077, Clone: 17A2) (right). Cells were not fixed.

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Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 488-conjugated Anti-Mouse CD3 (CL488-65077, Clone: 17A2) at dilution of 1:500.

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1X10^6 C57BL/6 mouse splenocytes were surface co-stained with PE Anti-Mouse CD4 (GK1.5) and 0.5 ug CoraLite® Plus 647 Anti-Mouse CD3 (CL647-65077, Clone:17A2) or 0.5 ug CoraLite® Plus 647 Rat IgG2b Isotype Control (LTF-2) (CL647-65211, Clone: LTF-2). Cells were not fixed.

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Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 647-conjugated Anti-Mouse CD3 (CL647-65077, Clone: 17A2) at dilution of 1:500.

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1X10^6 mouse splenocytes were surface stained with CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) (CL647-65104, Clone: GK1.5) and 0.5 ug CoraLite®555 Anti-Mouse CD3 (CL555-65077, Clone: 17A2) or 0.5 ug Isotype Control. Cells were not fixed.

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1X10^6 mouse splenocytes were surface co-stained with FITC Anti-Mouse CD4 and 0.5 ug CoraLite®594 Anti-Mouse CD3 (CL594-65077, Clone: 17A2) or 0.5 ug Isotype Control. Cells were not fixed.

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