1X10^6 C57BL/6 mouse splenocytes were surface stained with APC-Anti-Mouse CD3ε (APC-65060, Clone: 145-2C11) and 0.25 ug FITC-Rat IgG2b isotype control (left) or 0.25 ug FITC Anti-Mouse CD16 / CD32 (FITC-65080, Clone: 2.4G2) (right). Cells were not fixed.

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1x10^6 mouse splenocytes were stained with 0.5 ug Anti-Mouse CD16 / CD32 (65080-1-Ig, Clone: 2.4G2) and FITC anti-rat IgG2b Antibody at dilution 1:100, or 0.5 ug Control Antibody and FITC anti-rat IgG2b Antibody at dilution 1:100. Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD3. Cells were not fixed.

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1X10^6 mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 488 Anti-Mouse CD16 / CD32 (CL488-65080, Clone:2.4G2) (red) or 0.25 ug Control Antibody. Cells were not fixed.

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1X10^6 C57BL/6 mouse splenocytes were surface co-stained with FITC Plus Anti-Mouse CD3 (17A2) and 0.125 ug CoraLite® Plus 647 Anti-Mouse CD16 / CD32 (CL647-65080, Clone:2.4G2) or 0.125 ug CoraLite® Plus 647 Rat IgG2b Isotype Control (LTF-2). Cells were not fixed.

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1X10^6 C57BL/6 mouse splenocytes were surface stained with APC-Anti-Mouse CD3ε (APC-65060, Clone: 145-2C11) and 0.13 ug PE Anti-Mouse CD16 / CD32 (PE-65080, Clone: 2.4G2). Cells were not fixed.

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1X10^6 C57BL/6 mouse splenocytes were surface stained with APC-Anti-Mouse CD3ε (APC-65060, Clone: 145-2C11) and 0.13 ug PE-Rat IgG2b isotype control antibody. Cells were not fixed.

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