1X10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD45 (65087-1-Ig, Clone: 30-F11) and FITC anti-rat IgG2b Antibody at dilution 1:200 (green), or stained with 0.5 ug rat IgG2b isotype control antibody and FITC anti-rat IgG2b Antibody at dilution 1:200 (black). Cells were not fixed.