1X10^6 human PBMCs were surface stained with 5 ul FITC Anti-Human CD45 (FITC-65109, Clone:HI30) (red) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

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1X10^6 human PBMCs were surface stained with 5 ul PE Anti-Human CD45 (PE-65109, Clone:HI30) (red) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

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1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD45 (65109-1-Ig, Clone:HI30) or Mouse IgG1 Isotype Control and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:500. Cells were not fixed. Lymphocytes were gated.

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Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD45 antibody (65109-1-Ig, Clone: HI30 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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1X10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CD45 (APC-65109, Clone:HI30) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

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1X10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CD45 (APC-65109, Clone:HI30) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

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1X10^6 human PBMCs were surface stained with 5 ul Atlantic Blue™ Anti-Human CD45 (AB-65109, Clone:HI30), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

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1X10^6 human PBMCs were surface stained with 5 ul Atlantic Blue™ Anti-Human CD45 (AB-65109, Clone:HI30), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

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1X10^6 human PBMCs were surface stained with 5 ul Cardinal Red™ Anti-Human CD45 (CR-65109, Clone:HI30) (red) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

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1X10^6 human PBMCs were surface stained with 5 ul Cardinal Red™ Anti-Human CD45 (CR-65109, Clone:HI30) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

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1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD45 (CL405-65109, Clone:HI30), or CoraLite® Plus 405 Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

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1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD45 (CL405-65109, Clone:HI30), or CoraLite® Plus 405 Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

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1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD45 (CL488-65109, Clone: HI30) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

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Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CoraLite® Plus 488 CD45 antibody (CL488-65109, Clone: HI30 ) at dilution of 1:200.

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