1X10^6 human PBMCs were surface stained with CL750 Anti-Human CD45 and 5 ul CoraLite® Plus 594 Anti-Human CD3 (CL594-65112, Clone:Hit3a), or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

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1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 594 Anti-Human CD3 (CL594-65112, Clone:Hit3a), or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

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1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD3 (65112-1-Ig, Clone:Hit3a) or Mouse IgG2a Isotype Control and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:500, then CL750 Anti-Human CD45. Cells were not fixed. Lymphocytes were gated.

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1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD3 (65112-1-Ig, Clone:Hit3a) or Mouse IgG2a Isotype Control and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:500. Cells were not fixed. Lymphocytes were gated.

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1X10^6 human peripheral blood lymphocytes were surface stained with 0.25 ug APC-anti-human CD3 (APC-65112, clone Hit3a) (red) or 0.25 ug APC-mouse IgG2a isotype control (blue). Cells were not fixed.

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1X10^6 human PBMCs were surface stained with 5 ul Atlantic Blue™ Anti-Human CD3 (AB-65112, Clone:Hit3a), or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

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1X10^6 human PBMCs were surface stained with 5 ul Atlantic Blue™ Anti-Human CD3 (AB-65112, Clone:Hit3a), or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

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1X10^6 human PBMCs were surface stained with CL750 Anti-Human CD45 and 5 ul Cardinal Red™ Anti-Human CD3 (CR-65112, Clone:Hit3a), or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

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1X10^6 human PBMCs were surface stained with 5 ul Cardinal Red™ Anti-Human CD3 (CR-65112, Clone:Hit3a), or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

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1X10^6 human peripheral blood lymphocytes were surface stained with APC-Anti-Human CD19 (APC-65145, Clone: SJ25C1) and 5.00 ul CoraLite® Plus 488-conjugated Anti-Human CD3 (CL488-65112, Clone: Hit3a). Cells were not fixed.

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1X10^6 human peripheral blood lymphocytes were surface stained with APC-Anti-Human CD19 (APC-65145, Clone: SJ25C1) and CoraLite® Plus 488-conjugated Mouse IgG2a isotype control antibody. Cells were not fixed.

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1X10^6 human PBMCs were surface stained with CL750 Anti-Human CD45 and 5 ul CoraLite® Plus 555 Anti-Human CD3 (CL555-65112, Clone:Hit3a), or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

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1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 555 Anti-Human CD3 (CL555-65112, Clone:Hit3a), or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

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1X10^6 human peripheral blood lymphocytes were surface stained with FITC-Anti-Human CD19 (FITC-65145, Clone: SJ25C1) and 5.00 ul CoraLite®647-conjugated Anti-Human CD3 (CL647-65112, Clone: Hit3a). Cells were not fixed.

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1X10^6 human peripheral blood lymphocytes were surface stained with FITC-Anti-Human CD19 (FITC-65145, Clone: SJ25C1) and CoraLite®647-conjugated Mouse IgG2a isotype control antibody. Cells were not fixed.

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