1X10^6 BALB/C mouse splenocytes were surface co-stained with PE Anti-Mouse CD3 (17A2) and 0.25 ug CoraLite® Plus 488 Anti-Mouse CD4 (CL488-65141, Clone:RM4-5) or 0.25 ug CoraLite® Plus 488 Rat IgG2a Isotype Control (2A3). Cells were not fixed.

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Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 488-conjugated Anti-Mouse CD4 (CL488-65141, Clone: RM4-5) at dilution of 1:500.

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1X10^6 C57BL/6 mouse splenocytes were surface co-stained with APC Anti-Mouse CD3 (17A2) and 0.25 ug PE Anti-Mouse CD4 (PE-65141, Clone:RM4-5) or 0.25 ug PE Rat IgG2a Isotype Control. Cells were not fixed.

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1X10^6 mouse splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD3, and 0.2 ug Anti-Mouse CD4 (65141-1-Ig, Clone:RM4-5) and FITC-Goat anti-Mouse IgG at dilution 1:100 or 0.2 ug Isotype Control and FITC-Goat anti-Mouse IgG at dilution 1:100. Cells were not fixed.

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1X10^6 C57BL/6 mouse splenocytes were surface stained with FITC-Anti-Mouse CD3 (FITC-65077, Clone: 17A2) and 0.13 ug APC Anti-Mouse CD4 (APC-65141, Clone: RM4-5). Cells were not fixed.

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1X10^6 C57BL/6 mouse splenocytes were surface stained with FITC-Anti-Mouse CD3 (FITC-65077, Clone: 17A2) and 0.13 ug APC-Rat IgG2a isotype control antibody. Cells were not fixed.

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1X10^6 mouse splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD3 and 0.5 ug CoraLite® Plus 405 Anti-Mouse CD4 (CL405-65141, Clone: RM4-5) or 0.5 ug Isotype Control. Cells were not fixed.

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1X10^6 mouse splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD3 and 0.5 ug CoraLite® Plus 555 Anti-Mouse CD4 (CL555-65141, Clone: RM4-5) or 0.5 ug Isotype Control. Cells were not fixed.

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1X10^6 BALB/c mouse splenocytes were surface stained with 0.125 ug CoraLite® Plus 647 Anti-Mouse CD4 (CL647-65141, Clone: RM4-5) (red), or 0.125 ug Control Antibody. Cells were not fixed.

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Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 647-conjugated Anti-Mouse CD4 (CL647-65141, Clone: RM4-5) at dilution of 1:500.

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1X10^6 mouse splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD3 and 0.5 ug FITC Anti-Mouse CD4 (FITC-65141, Clone: RM4-5) or 0.5 ug Isotype Control. Cells were not fixed.

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