1X10^6 human PBMCs were surface stained with CoraLite® Plus 488 Anti-Human CD3 and 0.2 ug Anti-Human CD4 (65147-1-Ig, Clone:SK3) labeled with FlexAble CoraLite® Plus 750 Antibody Labeling Kit for Mouse IgG1 (KFA024) (right), or Mouse IgG1 Isotype Control (left). Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD4 (65147-1-Ig, Clone:SK3) labeled with FlexAble CoraLite® Plus 750 Antibody Labeling Kit for Mouse IgG1 (KFA024) (red), or Mouse IgG1 Isotype Control (blue). Cells were not fixed. Lymphocytes were gated.

1x10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CD4 (APC-65147, Clone:SK3) or Mouse IgG1 Isotype Control, and CL488-conjugated Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

1x10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CD4 (APC-65147, Clone:SK3) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface co-stained with CL750 Anti-Human CD3 and 5 ul Atlantic Blue™ Anti-Human CD4 (AB-65147, Clone:SK3), or Atlantic Blue™ Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface stained with 5 ul Atlantic Blue™ Anti-Human CD4 (AB-65147, Clone:SK3), or Atlantic Blue™ Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface co-stained with CL750 Anti-Human CD3 and 5 ul Cardinal Red™ Anti-Human CD4 (CR-65147, Clone:SK3), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface stained with 5 ul Cardinal Red™ Anti-Human CD4 (CR-65147, Clone:SK3), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface co-stained with CL750 Anti-Human CD3 and 5 ul CoraLite® Plus 405 Anti-Human CD4 (CL405-65147, Clone:SK3), or CoraLite® Plus 405 Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD4 (CL405-65147, Clone:SK3), or CoraLite® Plus 405 Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface co-stained with CoraLite® Plus 750 Anti-Human CD3 and 5 ul CoraLite® Plus 488 Anti-Human CD4 (CL488-65147, Clone:SK3) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD4 (CL488-65147, Clone:SK3) (red) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface co-stained with CL750 Anti-Human CD3 and 5 ul CoraLite® Plus 555 Anti-Human CD4 (CL555-65147, Clone:SK3), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 555 Anti-Human CD4 (CL555-65147, Clone:SK3), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.