1X10^6 human peripheral blood lymphocytes were surface stained with 1 ug Anti-Human CD3 (65148-1-Ig, Clone: SK7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 1 ug isotype control and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were not fixed.

1X10^6 human peripheral blood lymphocytes were surface stained with 0.25 ug APC-anti-human CD3 (APC-65148, clone SK7) (red) or 0.25 ug APC-mouse IgG1 isotype control (blue). Cells were not fixed.

1X10^6 human peripheral blood lymphocytes were surface stained with 5 ul CoraLite®488-conjugated Anti-Human CD3 (CL488-65148, Clone:SK7) (green), or 5 ul CoraLite®488-conjugated Mouse IgG1 isotype control (black). Cells were not fixed.

1X10^6 human peripheral blood lymphocytes were surface stained with 5 ul CoraLite®647-conjugated Anti-Human CD3 (CL647-65148, Clone:SK7) (blue), or 5 ul CoraLite®647-conjugated Mouse IgG1 isotype control (black). Cells were not fixed.

1X10^6 human peripheral blood lymphocytes were surface stained with 1 ug FITC-anti-human CD3 (FITC-65148, clone SK7) (red) or 1 ug FITC-mouse IgG1 isotype control (blue). Cells were not fixed.

1X10^6 human peripheral blood lymphocytes were surface stained with 0.25 ug PE-anti-human CD3 (PE-65148, clone SK7) (red) or 0.25 ug PE-mouse IgG1 isotype control (blue). Cells were not fixed.