1X10^6 mouse splenocytes were surface stained with CoraLite®488 Plus Anti-Mouse CD3 (CL488-65077, Clone: 17A2) and 1 ug CoraLite® Plus 647 Anti-Mouse CD4 (CL647-65199, Clone: RM4-4) or CoraLite® Plus 647-Conjugated rat IgG2b isotype control. Cells were not fixed.