1X10^6 C57BL/c mouse splenocytes were surface co-stained with APC Anti-Mouse CD3ε and 0.25 ug Anti-Mouse CD8a (65205-1-Ig, Clone:5H10-1) and FITC anti-rat IgG2b Antibody at dilution 1:100, or rat IgG2b Isotype Control and FITC anti-rat IgG2b Antibody at dilution 1:100. Cells were not fixed.