Various lysates were subjected to SDS PAGE followed by western blot with 22037-1-AP (BIM, BCL2L11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22037-1-AP (BIM, BCL2L11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).