Untreated and PNGase F-treated lysates of HL-60 cells were subjected to SDS PAGE followed by western blot with 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).
human spleen tissue were subjected to SDS PAGE followed by western blot with 60258-1-Ig (CD11c/Integrin alpha X Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60258-1-Ig (CD11c/Integrin Alpha X antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60258-1-Ig (CD11c/Integrin Alpha X antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD11c/Integrin Alpha X antibody (60258-1-Ig, Clone: 2F1C10 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD11c/Integrin Alpha X antibody (60258-1-Ig, Clone: 2F1C10 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).