Untreated and PNGase F-treated lysates of fetal human brain tissue and human spleen tissue were subjected to SDS PAGE followed by western blot with 66282-1-Ig (CD200 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

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Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66282-1-Ig (CD200 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66282-1-Ig (CD200 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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1X10^6 U-937 cells were stained with 0.2 ug Anti-Human CD200 (66282-1-Ig, Clone: 5F3D6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution of 1:1000 (green), or with 0.2 ug mouse IgG1 isotype control antibody and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution of 1:1000 (black). Cells were fixed with 90% MeOH.

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HEK-293 cells were subjected to SDS PAGE followed by western blot with 14057-1-AP (CD200 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14057-1-AP (CD200 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14057-1-AP (CD200 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD200 (98007-2-RR, Clone:230268E6) or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with APC Anti-Human CD19 (HIB19) (APC-65110, Clone: HIB19). Cells were not fixed. Lymphocytes were gated.

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1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD200 (98007-1-RR, Clone:230268E6) or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) and PE-conjugated Goat Anti-Rabbit IgG. Cells were then co-stained with APC Anti-Human CD19 (HIB19) (APC-65110, Clone: HIB19). Cells were not fixed. Lymphocytes were gated.

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1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD200 (98007-2-PBS, Clone:230268E6) or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with APC Anti-Human CD19 (HIB19) (APC-65110, Clone: HIB19). Cells were not fixed. Lymphocytes were gated.

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1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD200 (98007-1-PBS, Clone:230268E6) or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) and PE-conjugated Goat Anti-Rabbit IgG. Cells were then co-stained with APC Anti-Human CD19 (HIB19) (APC-65110, Clone: HIB19). Cells were not fixed. Lymphocytes were gated.

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Untreated and PNGase F-treated lysates of fetal human brain tissue and human spleen tissue were subjected to SDS PAGE followed by western blot with 66282-1-Ig (CD200 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66282-1-PBS in a different storage buffer formulation.

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Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66282-1-Ig (CD200 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66282-1-PBS in a different storage buffer formulation.

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Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66282-1-Ig (CD200 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66282-1-PBS in a different storage buffer formulation.

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1X10^6 U-937 cells were stained with 0.2 ug Anti-Human CD200 (66282-1-Ig, Clone: 5F3D6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution of 1:1000 (green), or with 0.2 ug mouse IgG1 isotype control antibody and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution of 1:1000 (black). Cells were fixed with 90% MeOH. This data was developed using the same antibody clone with 66282-1-PBS in a different storage buffer formulation.

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Sandwich ELISA standard curve ofMP50062-1, CD200 Monoclonal Matched Antibody Pair - BSA and Azide free. Capture antibody: 68700-1-PBS. Detection antibody: 68700-2-PBS. Standard: Eg0006. Range: 31.3-2000 pg/mL.

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